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Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM). | LitMetric
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Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM).

Maria S Kuyukina Irena B Ivshina Irina O Korshunova Ekaterina V Rubtsova

J Microbiol Methods

Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia; Microbiology and Immunology Department, Perm State University, 15 Bukirev Street, 614900 Perm, Russia.

Published: December 2014

Using combined confocal laser scanning and atomic force microscopy (CLSM/AFM), bacterial viability under organic solvent stress was assessed at single cell level. Solvent-exposed bacteria stained with the LIVE/DEAD BacLight fluoresced green or red, allowing viable and dead cell discrimination. However, with toluene, butanol and acetonitrile, dually fluorescent cells appeared having compromised cell membranes. Changes in size, surface/volume ratio and roughness were revealed as possible resistance mechanisms.

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http://dx.doi.org/10.1016/j.mimet.2014.08.020DOI Listing

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