AI Article Synopsis
- A Love Wave immunosensor was created to detect carbaryl pesticide using advanced electronic and automated systems that ensure high sensitivity and effectiveness.
- The sensor operates on a competitive immunoassay format with a sturdy monoclonal antibody and can be reused for at least 70 assays without major signal loss.
- It demonstrates a limit of detection of 0.09 μg/L and operates effectively within a linear range, making it a strong candidate for monitoring pesticide levels in drinking water according to European standards.
Article Abstract
A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14-1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208181 | PMC |
| http://dx.doi.org/10.3390/s140916434 | DOI Listing |
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