A novel high-resolution melting analysis-based method for Yersinia enterocolitica genotyping.

J Microbiol Methods

Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Brazil. Electronic address:

Published: November 2014

Pathogenic Yersinia enterocolitica strains are associated with biotypes 1B, 2-5, while environmental strains with biotype 1A. In this work a method for Y. enterocolitica genotyping based on HRMA to determine SNPs was developed and the genetic diversity of 50 strains was determined. The strains were clustered into three groups consistent with the pathogenic profile of each biotype. The results provided a better understanding of the Y. enterocolitica genetic variability.

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http://dx.doi.org/10.1016/j.mimet.2014.08.011DOI Listing

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