During four years in our laboratory work a small number of bacteriologically confirmed tuberculous pleurises was observed. By different procedure of inoculation of Löwenstein-Jensen medium the greater number of clinical diagnosis was confirmed. To avoid negative influence of the base and the acid on Mycobacterium tuberculosis which could be found in smaller number in pleural punctate, inoculation of the bioptic material was performed directly on medium without prior digestion and decontamination. Our apprehension that by direct inoculation the number of contaminations of media might be increased was not correct. On the contrary, first results in this study reveal that by this procedure a greater number of positive results has been obtained.

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