AI Article Synopsis
- A new liquid chromatography method combined with mass spectrometry has been created to accurately measure the levels of the blood thinners dabigatran and rivaroxaban.
- The method utilized stable isotope-labeled standards for precise quantification and involved a straightforward protein precipitation step for preparing plasma samples.
- Validation showed the method is reliable across various parameters, including sensitivity with a lower limit of quantification set at 2.5ng/mL for both medications.
Article Abstract
A sensitive and accurate liquid chromatography method with mass spectrometry detection was developed and validated for the quantification of dabigatran (Pradaxa(®)) and rivaroxaban (Xarelto(®)). (13)C6-dabigatran and (13)C6-rivaroxaban were used as the internal standard. A single-step protein precipitation was used for plasma sample preparation. This method was validated with respect to linearity, selectivity, inter- and intra-day precision and accuracy, limit of quantification and stability. The lower limit of quantification was 2.5ng/mL for both drugs in plasma.
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| http://dx.doi.org/10.1016/j.jpba.2014.08.011 | DOI Listing |
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