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Authentication of pineapple (Ananas comosus [L.] Merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (HS-SPME-GC-SIM-MS). | LitMetric

AI Article Synopsis

  • Researchers used HS-SPME-GC-SIM-MS to measure specific lactones in pineapples, including δ-lactones and γ-lactones, for quality analysis.
  • A stable isotope dilution assay (SIDA) was employed to quantitatively analyze pineapple lactones at different ripeness stages after harvest, focusing on fully ripe air-freighted and green-ripe sea-freighted fruits.
  • While γ-C6 lactone maintained high enantiomeric purity in freshly harvested pineapples, its purity decreased in sea-freighted fruits during storage, highlighting the importance of chiroanalysis for fruit quality assessment.

Article Abstract

Headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (HS-SPME-GC-SIM-MS) allowed quantitative determination of δ-lactones (δ-C8, δ-C10) and γ-lactones (γ-C6, γ-C8, γ-C10). A stable isotope dilution assay (SIDA) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted fruits, covering the relevant shelf-life of the fruits. Fresh pineapples harvested at full maturity were characterised by γ-C6 of high enantiomeric purity remaining stable during the whole post-harvest period. In contrast, the enantiomeric purity of γ-C6 significantly decreased during post-harvest storage of sea-freighted pineapples. The biogenetical background and the potential of chirospecific analysis of lactones for authentication and quality evaluation of fresh pineapple fruits are discussed.

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Source
http://dx.doi.org/10.1016/j.foodchem.2014.07.071DOI Listing

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