We used real-time polymerase chain reaction and culture to demonstrate persistent colonization of soils by Coccidioides immitis, an agent of valley fever, in Washington State linked to recent human infections and located outside the endemic range. Whole-genome sequencing confirmed genetic identity between isolates from soil and one of the case-patients.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296125PMC
http://dx.doi.org/10.1093/cid/ciu681DOI Listing

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