AI Article Synopsis
- - Investigated the use of short interfering duplexes with arabinonucleotides (ANA) and their modified versions, finding that ANA fits well in siRNA strands and can be altered without losing effectiveness.
- - Combining ANA with RNA and 2'F-ANA in specific patterns can lead to siRNA duplexes that maintain or even enhance their effectiveness compared to traditional siRNA.
- - Successful application of modified duplexes was tested using firefly luciferase in HeLa cells and further utilized to target down-regulated in renal cell carcinoma (DRR) for potential glioblastoma treatment.
Article Abstract
We have investigated, for the first time, short interfering duplexes containing arabinonucleotides (ANA; the 2'-stereoisomer of RNA), as well as combinations of ANA with RNA, and their 2'-fluorinated derivatives 2F-ANA and/or 2'F-RNA. The results show that ANA is especially well accommodated in the sense strand of small interfering RNA (siRNA) duplexes, which can be extensively modified with little effect on potency. Furthermore, combining ANA with RNA and 2'F-ANA in siRNA passenger strands, particularly in patterns that bias duplex thermal stability, produces duplexes with similar (and sometimes enhanced) potency compared with native siRNA. Effective patterns of modification were identified against firefly luciferase screens in HeLa cells and then applied to knockdown of down-regulated in renal cell carcinoma (DRR), a novel and clinically tractable target for the treatment of glioblastoma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/nat.2014.0496 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!