AI Article Synopsis
- Graphene is being explored as a valuable substrate for stem cell culture, particularly enhancing the differentiation of human embryonic stem cells (hESCs) into heart cells.
- The study highlights that the nanoroughness of graphene contributes to this enhanced differentiation, making it a promising option for stem cell therapy development.
- Coating graphene with vitronectin is essential for maintaining high cell viability, and the results show that hESCs on graphene express genes linked to heart cell development more effectively than those on traditional substrates like glass or Matrigel.
Article Abstract
Graphene has drawn attention as a substrate for stem cell culture and has been reported to stimulate the differentiation of multipotent adult stem cells. Here, we report that graphene enhances the cardiomyogenic differentiation of human embryonic stem cells (hESCs) at least in part, due to nanoroughness of graphene. Large-area graphene on glass coverslips was prepared via the chemical vapor deposition method. The coating of the graphene with vitronectin (VN) was required to ensure high viability of the hESCs cultured on the graphene. hESCs were cultured on either VN-coated glass (glass group) or VN-coated graphene (graphene group) for 21 days. The cells were also cultured on glass coated with Matrigel (Matrigel group), which is a substrate used in conventional, directed cardiomyogenic differentiation systems. The culture of hESCs on graphene promoted the expression of genes involved in the stepwise differentiation into mesodermal and endodermal lineage cells and subsequently cardiomyogenic differentiation compared with the culture on glass or Matrigel. In addition, the culture on graphene enhanced the gene expression of cardiac-specific extracellular matrices. Culture on graphene may provide a new platform for the development of stem cell therapies for ischemic heart diseases by enhancing the cardiomyogenic differentiation of hESCs.
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| http://dx.doi.org/10.1016/j.bbrc.2014.08.062 | DOI Listing |
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