Microscopy assays for evaluation of mast cell migration and chemotaxis.

Methods Mol Biol

Laboratory of Signal Transduction Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic.

Published: April 2015

A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.

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http://dx.doi.org/10.1007/978-1-4939-1173-8_12DOI Listing

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