Amphiphilic tris-urea molecules self-assemble into a supramolecular hydrogel in tris(hydroxymethyl)aminomethane-glycine buffer. The supramolecular hydrogel is used as a matrix for the electrophoresis of acidic native proteins, in which proteins are separated based on their isoelectric points rather than their molecular weights. The proteins remain in their native forms during migration, and their activities are retained after electrophoresis. Glucoside substituents on the amphiphilic tris-urea molecule allow for the affinity electrophoresis of a carbohydrate-binding protein to be performed. The proteins can be efficiently recovered from the supramolecular hydrogel using a simple procedure. This is a major advantage of using this noncovalent, self-assembled material.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ac5026539 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication of Hypoxia-Mimicking Supramolecular Hydrogels for Cartilage Repair.
ACS Appl Bio Mater
January 2025
Regenerative Medicine and Stem Cell Laboratory (RMS), Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi 502 284, Telangana, India.
Despite advancements in chronic arthritis treatment, there remains a significant demand for advanced nanotechnologies capable of efficiently delivering a wide range of therapeutic agents to provide symptomatic relief and facilitate the healing of inflamed cartilage tissue. Considering the significant impact of hypoxia on the development and maintenance of chondral tissue, replicating its effects on stem cells could be a potential approach for the treatment of osteoarthritis (OA). Cobalt is a prominent hypoxia-inducing agent, owing to its ability to activate the hypoxia-inducible factor (HIF) pathway regardless of cellular oxygen levels.
View Article and Find Full Text PDFRecent Progress in Self-Healing Triboelectric Nanogenerators for Artificial Skins.
Biosensors (Basel)
January 2025
Henan Energy Conversion and Storage Materials Engineering Center, College of Science, Henan University of Engineering, Zhengzhou 451191, China.
Self-healing triboelectric nanogenerators (TENGs), which incorporate self-healing materials capable of recovering their structural and functional properties after damage, are transforming the field of artificial skin by effectively addressing challenges associated with mechanical damage and functional degradation. This review explores the latest advancements in self-healing TENGs, emphasizing material innovations, structural designs, and practical applications. Key materials include dynamic covalent polymers, supramolecular elastomers, and ion-conductive hydrogels, which provide rapid damage recovery, superior mechanical strength, and stable electrical performance.
View Article and Find Full Text PDFInfluence of Electrostatic Interactions on the Self-Assembly of Charged Peptides.
Gels
January 2025
Faculty of Medicine, Dalian University of Technology, Dalian 116033, China.
Peptides can be designed to self-assemble into predefined supramolecular nanostructures, which are then employed as biomaterials in a range of applications, including tissue engineering, drug delivery, and vaccination. However, current self-assembling peptide (SAP) hydrogels exhibit inadequate self-healing capacities and necessitate the use of sophisticated printing apparatus, rendering them unsuitable for 3D printing under physiological conditions. Here, we report a precisely designed charged peptide, Z5, with the object of investigating the impact of electrostatic interactions on the self-assembly and the rheological properties of the resulting hydrogels.
View Article and Find Full Text PDFSynergistic Antibacterial Action of Norfloxacin-Encapsulated G4 Hydrogels: The Role of Boronic Acid and Cyclodextrin.
Gels
January 2025
"Petru Poni" Institute of Macromolecular Chemistry, Romanian Academy, Centre of Advanced Research in Bionanoconjugates and Biopolymers, Grigore Ghica Voda Alley 41 A, 700487 Iasi, Romania.
In this present study, we developed and characterized a series of supramolecular G4 hydrogels by integrating -cyclodextrin (-CD) and boronic acid linkers into a supramolecular matrix to enhance antibacterial activity against (). We systematically investigated how varying the number of free boronic acid moieties (ranging from two to six), along with guanosine and β-CD content, influences both the structural integrity and antimicrobial efficacy of these materials. Comprehensive characterization using FTIR, circular dichroism, X-ray diffraction, SEM, AFM, and rheological measurements confirmed successful synthesis and revealed that higher boronic acid content correlated with a stronger, more organized network.
View Article and Find Full Text PDFBio-Inspired Highly Stretchable and Ultrafast Autonomous Self-Healing Supramolecular Hydrogel for Multifunctional Durable Self-Powered Wearable Devices.
Small
January 2025
Institute of Biomass and Function Materials & National Demonstration Centre for Experimental Light Chemistry Engineering Education, College of Bioresources Chemistry and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, P. R. China.
As skin bioelectronics advances, hydrogel wearable devices have broadened perspectives in environment sensing and health monitoring. However, their application is severely hampered by poor mechanical and self-healing properties, environmental sensitivity, and limited sensory functions. Herein, inspired by the hierarchical structure and unique cross-linking mechanism of hagfish slime, a self-powered supramolecular hydrogel is hereby reported, featuring high stretchability (>2800% strain), ultrafast autonomous self-healing capabilities (electrical healing time: 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!