Background: Some genetic and phenotypic variables are associated among distinct microbial populations.

Objectives: The associations between multi-drug resistance (MDR) phenotypes, prevalence of antibiotic resistance integrons (ARIs), bla SHV, bla TEM and bla CTX-M gene carriage and genetic fingerprints of random amplified polymorphic DNA (RAPD), confirmed by pulsed field gel electrophoresis (PFGE), were investigated among extended-spectrum β-lactamases (ESBL)-producing nosocomial isolates of Klebsiella pneumoniae.

Materials And Methods: Susceptibility of 35 ESBL-producing K. pneumoniae nosocomial isolates to 22 antimicrobial agents was determined. Integron carriage was detected using specific primers for intI1, intI2 and intI3 genes by PCR.

Results: All isolates were resistant to piperacillin and susceptible to imipenem. MDR phenotype was observed in 91.4% of the isolates. Class 1 integrons were detected in 21 (60%) and class 2 integrons in 3 (8.57%) of the isolates. Two of the isolates carried both classes and none harbored class 3 integrons. Significant correlations were observed between resistance to aminoglycosides, fluoroquinolones and sulfonamides, and between genotype groups with carriage of ARIs, MDR phenotype and bla SHV gene carriage. ARI carriage was also significantly associated with MDR phenotype.

Conclusions: Our findings suggest the possible co-carriage of some bla SHV genes and ARIs on the same plasmids harboring the MDR genes. Possible role of integrons in dissemination of ESBL-encoding bla SHV genes among ESBL-producing K. pneumoniae nosocomial isolates may be inferred.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138679PMC
http://dx.doi.org/10.5812/jjm.8747DOI Listing

Publication Analysis

Top Keywords

bla shv
16
gene carriage
12
nosocomial isolates
12
class integrons
12
multi-drug resistance
8
carriage genetic
8
genetic fingerprints
8
esbl-producing pneumoniae
8
pneumoniae nosocomial
8
mdr phenotype
8

Similar Publications

Klebsiella pneumoniae is an opportunistic pathogen responsible for various infections in humans and animals. It is known for its resistance to multiple antibiotics, particularly through the production of Extended-Spectrum Beta-Lactamases (ESBLs), and its ability to form biofilms that further complicate treatment. This study aimed to isolate and identify K.

View Article and Find Full Text PDF

Genomic Analysis of Virulent, Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca from Bloodstream Infections, South Africa.

Microb Pathog

January 2025

Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa; School of Pharmacy, University of Jordan, Amman 11942, Jordan.

Unlabelled: The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates.

Methods: A total of 46 suspected Klebsiella species (spp.) were collected from blood cultures within the uMgungundlovu District in the KwaZulu-Natal Province.

View Article and Find Full Text PDF

Background: Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium capable of causing severe infections in immunocompromised patients such as those suffering from chronic kidney disease (CKD). This study aimed to determine the resistance profile of Pseudomonas aeruginosa, and the prevalence of extended-spectrum β-lactamase (ESBL) resistance genes in patients with chronic kidney disease.

Methods: The prevalence of Pseudomonas aeruginosa was investigated in 458 patients, including 197 CKD patients and 261 patients suffering from gastrointestinal infections.

View Article and Find Full Text PDF

CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand.

J Infect Public Health

December 2024

Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address:

Background: Currently, antimicrobial agents are widely used in both animals and agriculture, causing the crisis of multidrug-resistant (MDR) bacteria. In this study we surveyed for 4 important antimicrobial-resistant bacteria: extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae and Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA) from the environment around chicken and pig farms.

View Article and Find Full Text PDF

Background And Objectives: is a common pathogen associated with healthcare-related infections. It is particularly notable for its ability to develop resistance to multiple antibiotics, making treatment challenging. During the COVID-19 pandemic, increased antibiotic use to manage critically ill patients was contributed to the rise of multidrug-resistant .

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!