Interactions between fungi and prokaryotes are abundant in many ecological systems. A wide variety of biomolecules regulate such interactions and many of them have found medicinal or biotechnological applications. However, studying a fungal-bacterial system at a cellular level is technically challenging. New microfluidic devices provided a platform for microscopic studies and for long-term, time-lapse experiments. Application of these novel tools revealed insights into the dynamic interactions between the basidiomycete Coprinopsis cinerea and the bacterium Bacillus subtilis. Direct contact was mediated by polar attachment of bacteria to only a subset of fungal hyphae suggesting a differential competence of fungal hyphae and thus differentiation of hyphae within a mycelium. The fungicidal activity of B. subtilis was monitored at a cellular level and showed a novel mode of action on fungal hyphae.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c4ib00154k | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antifungal activity of essential oils and their potential synergistic effect with amphotericin B.
Sci Rep
December 2024
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Candida albicans is a common opportunistic pathogen, causing infections ranging from superficial to bloodstream infections. The limited antifungal options and rising drug resistance challenge clinical treatment. We screened 98 essential oils and identified 48 with antifungal activity against Candida albicans at 1% concentration, determining their minimum inhibitory concentrations (MIC).
View Article and Find Full Text PDFExploring the anti-biofilm and gene regulatory effects of anti-inflammatory drugs on Candida albicans.
Naunyn Schmiedebergs Arch Pharmacol
December 2024
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Researchers have repurposed several existing anti-inflammatory drugs as potential antifungal agents in recent years. So, this study aimed to investigate the effects of anti-inflammatory drugs on the growth, biofilm formation, and expression of genes related to morphogenesis and pathogenesis in Candida albicans. The minimum inhibitory concentration (MIC) of anti-inflammatory drugs was assessed using the broth microdilution method.
View Article and Find Full Text PDFUpregulation of ACC deaminase gene in Bacillus velezensis UTB96 improved yield and shelf Life of Agaricus bisporus.
Sci Rep
December 2024
Department of Plant Protection, College of Agriculture, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran.
Agaricus bisporus is globally a most extensively consumed species of edible mushrooms. Ethylene secreted by A. bisporus mycelium suppress the initiation of fructification.
View Article and Find Full Text PDFSaprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region.
View Article and Find Full Text PDFFabrication of Lab-on-a-Chip Devices to Study the Forces Imparted by Growing Phytophthora Hyphae.
Methods Mol Biol
December 2024
Biomolecular Interaction Centre, University of Canterbury, Christchurch, New Zealand.
In this chapter, we describe the design and manufacture of a Lab-on-a-Chip (LoC) device suitable for measuring the μN forces exerted by tips of growing Phytophthora hyphae. LoC describes microfluidic devices, typically made of the polymer polydimethylsiloxane (PDMS), that are increasingly being used to answer fundamental questions in biological, chemical, physical, and medical research. These LoC devices enable the integration of several laboratory functions on small plastic devices that are quick to produce and easy to replicate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!