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Fluorescent microspheres are at the forefront of biosensing technologies. They can be used for a wide range of biomedical applications. They consist of organic dyes and polymers, which are relatively immune to photobleaching and other environmental factors.

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Objective: Neutrophil gelatinase-associated lipocalin (NGAL) has been identified by the International Nephrology Association (INA) as a promising biomarker for the early evaluation of renal injury. This study aimed to develop and evaluate NGAL test strips as a rapid, simple, and economical method for the early diagnosis of acute kidney injury (AKI).

Methods: Recombinant prokaryotic expression vectors, purified NGAL protein, and anti-NGAL monoclonal antibodies were prepared.

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Fluorescent strip sensor for rapid and ultrasensitive determination of fluoroquinolones in fish and milk.

Analyst

January 2023

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

The synthetic antibiotics fluoroquinolones are popular due to their good antibacterial performance and low price, but the risk to human health caused by their residues has attracted great attention. In this study, an ultra-sensitive mAb, 4D7, was prepared with an IC of 0.027 ng mL to norfloxacin (NOR) and cross-reactivity of 19.

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Methyl parathion (MP) residues have aroused extensive attention on account of their significant threat to the environment and food safety. Currently reported fluorescent methods used for MP sensing largely depend upon an enzyme. Designing a facile and specific enzyme-free MP fluorescent sensor is in great demand, which remains a challenge.

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Carbon dot-functionalized macroporous adsorption resin for bifunctional ultra-sensitive detection and fast removal of iron(III) ions.

Anal Methods

October 2022

School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, China.

Heavy metal pollution has spread around the world with the development of industry, posing a major threat to human health. It is urgent to design and fabricate bifunctional materials for detection and adsorption of heavy metal ions. Herein, poly(glycidyl methacrylate--ethylene glycol dimethacrylate) microspheres, a kind of common macroporous adsorption resin (MAR), were employed as the matrix, and carbon dots (CDs) with excellent optical properties were grafted onto the surface of MAR by surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated "thiol-yne" click chemistry.

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