Bacteria-mediated aerobic degradation of hexacosane in vitro conditions.

Bioresour Technol

Plant Ecology & Environmental Science Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India. Electronic address:

Published: October 2014

AI Article Synopsis

  • Research investigated how two bacteria, Pseudomonas sp. BP10 and Stenotrophomonas nitritireducens E9, break down hexacosane, a long-chain alkane, in a controlled environment.
  • Both bacteria showed high degradation rates, achieving 82% individually and 98% when working together, suggesting teamwork enhances their effectiveness.
  • The study also noted biochemical changes, like increased cell surface hydrophobicity, indicating how the bacteria utilize hexacosane for growth while producing specific enzymes involved in its breakdown.

Article Abstract

In vitro degradation of hexacosane (C26H54), a HMW n-alkane, was studied in MSM by two bacterial strains i.e., Pseudomonas sp. BP10 and Stenotrophomonas nitritireducens E9, isolated from petroleum sludge, in isolation and combination. The results revealed that both the strains were able to metabolize hexacosane by 82% in isolation and 98% in their consortium after 7days. An enhancement of 16% in hexacosane degradation by the consortium indicated an additive action of bacterial strains. However, in control, a degradation of 21% was attributed to abiotic factors. During incubation with hexacosane, both the bacteria continued to multiply in isolation and consortium, which reflected that hexacosane was utilized by bacteria as a carbon and energy source. Activities of alkane hydroxylase and alcohol dehydrogenase were differentially expressed in isolation and combination, indicating their involvement in hexacosane degradation. Enhanced cell surface hydrophobicity and emulsification index and reduced surface tension also supported the degradation process.

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Source
http://dx.doi.org/10.1016/j.biortech.2014.07.091DOI Listing

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