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Isolation of a Novel Low-Temperature-Active and Organic-Solvent-Stable Mannanase from the Intestinal Metagenome of .
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Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
The black soldier fly, , is a voracious scavenger of various organic materials; therefore, it could be exploited as a biological system for processing daily food waste. In order to survey novel hydrolytic enzymes, we constructed a fosmid metagenome library using unculturable intestinal microorganisms from . Through functional screening of the library on carboxymethyl cellulose plates, we identified a fosmid clone, the product of which displayed hydrolytic activity.
View Article and Find Full Text PDFAutoregulation of the glial gene reversed polarity in Drosophila.
Sci Rep
January 2025
Department of Biology, The University of Mississippi, University, MS, 38677, USA.
During development, cells of the nervous system begin as unspecified precursors and proceed along one of two developmental paths to become either neurons or glia. Work in the fruit fly Drosophila melanogaster has established the role of the transcription factor Glial cells missing (Gcm) in directing neuronal precursor cells to assume a glial cell fate. Gcm acts on many target genes, one of which is reversed polarity (repo).
View Article and Find Full Text PDFCompromising tyrosine hydroxylase function extends and blunts the temporal profile of reinforcement by dopamine neurons in .
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Leibniz Institute for Neurobiology (LIN), Department of Genetics of Learning and Memory, Magdeburg, 39118 Germany
For a proper representation of the causal structure of the world, it is adaptive to consider both evidence for and evidence against causality. To take punishment as an example, the causality of a stimulus is unlikely if there is a temporal gap before punishment is received, but causality is credible if the stimulus immediately precedes punishment. In contrast, causality can be ruled out if the punishment occurred first.
View Article and Find Full Text PDFEffects of black soldier fly larvae oil on lipid metabolism, liver fatty acid composition, and plasma metabolite profiles in gilthead seabream juveniles.
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Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, Ed. FC4, 4169-007 Porto, Portugal.
The potential of insects as alternative ingredients in animal feeds is well-established. However, limited information is available on the use of insect oils as alternative lipid sources in aquafeeds. To address this, a study was conducted on gilthead seabream (Sparus aurata) juveniles to evaluate the effects of including black soldier fly (Hermetia illucens) larvae oil (HIO).
View Article and Find Full Text PDFSplit-Cre-mediated GFP expression as a permanent marker for flagellar fusion of in its tsetse fly host.
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Institute of Cell Biology, University of Bern, Bern, Switzerland.
Unlabelled: Trypanosomes have different ways of communicating with each other. While communication via quorum sensing, or by the release and uptake of extracellular vesicles, is widespread in nature, the phenomenon of flagellar fusion has only been observed in . We showed previously that a small proportion of procyclic culture forms (corresponding to insect midgut forms) can fuse their flagella and exchange cytosolic and membrane proteins.
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