AI Article Synopsis
- Researchers used PCR to amplify and sequence the genome of human bocavirus 2 (HBoV2) from fecal samples collected in 2010, resulting in a 5444 bp genome (HBoV2-NC).
- Phylogenetic analysis indicated that HBoV2-NC is most closely related to the HBoV2 Lanzhou strain.
- The study also predicted that HBoV2-NC contains inverted terminal repeats with a typical stem-loop structure, which is characteristic of other parvoviruses, and some additional flanking sequences were identified through linker-PCR.
Article Abstract
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
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