Remodeling cell surface glycans using glycosylation inhibitors.

Cell surface glycan remodeling is a useful method to modulate glycan-lectin interactions. In this chapter, a facile and reliable method to remodel mammalian cell surface N-glycans using inhibitors for N-glycan-processing enzymes is described. The method is widely applicable to many mammalian systems because those inhibitors work for the conserved glycosylation pathways among species.