AI Article Synopsis
- * Treatments involved exposing the bacteria to rifampicin and mitomycin for 36 hours, followed by RNA extraction and analysis using qRT-PCR to measure gene expression.
- * Findings showed that the MDR strain displayed a quicker and stronger response in recA expression compared to the drug-susceptible strain, indicating distinct DNA repair mechanisms between the two types.
Article Abstract
Objectives: To investigate and compare the expression of recA and recX, components of the SOS pathway, following rifampicin treatment in drug-susceptible and MDR clinical strains of Mycobacterium tuberculosis.
Methods: Strains (M. tuberculosis and Mycobacterium smegmatis) were subjected to rifampicin- and mitomycin-induced stress for 36 h followed by RNA extraction. recA and recX in the RNA extract were estimated using qRT-PCR.
Results: The MDR clinical strain induced faster (24 h) and higher (7-fold) levels of recA as compared with the drug-susceptible strain (36 h) in response to rifampicin. recX levels were found to rise with an increase in levels of recA; however, the levels were relatively higher than recA.
Conclusions: Drug-susceptible and MDR strains have different kinetics of induction of DNA repair.
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| http://dx.doi.org/10.1093/jac/dku319 | DOI Listing |
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