AI Article Synopsis

  • The project analyzed cerebrospinal fluid proteomic profiles from Multiple Sclerosis (MS) patients at different clinical stages to identify unique characteristics for various MS types.
  • 24 Clinically Isolated Syndrome (CIS), 16 Relapsing Remitting (RR) MS, and 11 Progressive (Pr) MS patients' samples were examined using MALDI-TOF mass spectrometry, revealing 348 significant peak signals.
  • Notable findings included upregulation of certain proteins in RRMS compared to PrMS and a unique intensity peak distinguishing CIS from RRMS, suggesting the potential value of this technique in understanding MS pathology, despite limitations in sample size and statistical significance.

Article Abstract

The aim of our project was to perform an exploratory analysis of the cerebrospinal fluid (CSF) proteomic profiles of Multiple Sclerosis (MS) patients, collected in different phases of their clinical course, in order to investigate the existence of peculiar profiles characterizing the different MS phenotypes. The study was carried out on 24 Clinically Isolated Syndrome (CIS), 16 Relapsing Remitting (RR) MS, 11 Progressive (Pr) MS patients. The CSF samples were analysed using the Matrix Assisted Laser Desorption Ionisation Time Of Flight (MALDI-TOF) mass spectrometer in linear mode geometry and in delayed extraction mode (m/z range: 1000-25000 Da). Peak lists were imported for normalization and statistical analysis. CSF data were correlated with demographic, clinical and MRI parameters. The evaluation of MALDI-TOF spectra revealed 348 peak signals with relative intensity ≥ 1% in the study range. The peak intensity of the signals corresponding to Secretogranin II and Protein 7B2 were significantly upregulated in RRMS patients compared to PrMS (p<0.05), whereas the signals of Fibrinogen and Fibrinopeptide A were significantly downregulated in CIS compared to PrMS patients (p<0.04). Additionally, the intensity of the Tymosin β4 peak was the only signal to be significantly discriminated between the CIS and RRMS patients (p = 0.013). Although with caution due to the relatively small size of the study populations, and considering that not all the findings remained significant after adjustment for multiple comparisons, in our opinion this mass spectrometry evaluation confirms that this technique may provide useful and important information to improve our understanding of the complex pathogenesis of MS.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123901PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0103984PLOS

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