Evaluation of the photobiomodulation in L929 cell culture.

Exp Biol Med (Maywood)

Laboratory of Tissue and Cell Biology, Development and Research Institute, Universidade do Vale do Paraíba (UNIVAP), Urbanova, 12244-000 São José dos Campos, Brazil.

Published: December 2014

AI Article Synopsis

  • Low-level laser therapy is used for bio-modulation and can affect cell behavior in either stimulating or inhibitory ways based on specific irradiation conditions.
  • The study focused on the L929 cell line, testing various wavelengths (685 and 830 nm), energy densities (0.1 to 30 J/cm²), and serum concentrations to observe cell response to laser irradiation.
  • Results indicated that energy densities between 5 to 30 J/cm² stimulated cell growth, while lower densities (0.1 to 3 J/cm²) inhibited cell activity, with no significant effect from varying fetal bovine serum concentrations on cell response.

Article Abstract

Low-level laser therapy has become an important tool for bio-modulation process. It can induce stimulatory or inhibitory effects according to cell behavior at specific irradiation. Our objective was to determine L929 cell line response to irradiation at λ 685 and 830 nm, concentrations of 5 and 10% fetal bovine serum and different energy densities of 0.1, 0.5, 1, 2, 3, 5, 7, 10, 20, and 30 J/cm(2). Thus, cells were plated at 1 × 10(5) cells/mL and irradiated with semiconductor laser As-Ga-Al. Twenty-four hours after irradiation, cells were subjected to MTT, neutral red, crystal violet tests, and cell staining was performed using the kit Alexa Fluor 488 Annexin V-FITC and propidium iodide. Our results showed that low-level laser therapy stimulates effect when the energy density is 5 to 3030 J/cm(2) and inhibits effects on energy density 0.1 to 3 J/cm(2). This inhibitory effect was evidenced by the absence of dead cells labeled, decreased cell density, and by the absorption of neutral red in intact cells. The study also demonstrated that fetal bovine serum, at different concentrations, did not affect response of the cells after irradiation.

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http://dx.doi.org/10.1177/1535370214542070DOI Listing

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