In this study a simple protocol was developed for purifying phycocyanin (PC) from (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A/A). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A/A) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).
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| http://dx.doi.org/10.1007/s40502-014-0094-7 | DOI Listing |
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