Glutathione S-transferases (GSTs), are a family of enzymes belonging to the phase II metabolism that catalyse the formation of thioether conjugates between the endogenous tripeptide glutathione and xenobiotic compounds. The voltammetric behaviour of glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione S-transferase (GST), as well as the catalytic conjugation reaction of GSH to CDNB by GST was investigated at room temperature, T=298.15K (25°C), at pH6.5, for low concentration of substrates and enzyme, using differential pulse (DP) voltammetry at a glassy carbon electrode. Only GSH can be oxidized; a sensitivity of 0.14nA/μM and a LOD of 6.4μM were obtained. The GST kinetic parameter electrochemical evaluation, in relation to its substrates, GSH and CDNB, using reciprocal Michaelis-Menten and Lineweaver-Burk double reciprocal plots, was determined. A value of KM~100μM was obtained for either GSH or CDNB, and Vmax varied between 40 and 60μmol/min per mg of GST.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bioelechem.2014.07.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spontaneous reversal of small molecule-induced mitochondrial uncoupling: the case of anilinothiophenes.
FEBS J
December 2024
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia.
Tissue specificity can render mitochondrial uncouplers more promising as leading compounds for creating drugs against serious diseases. In search of tissue-specific uncouplers, we address anilinothiophenes as possible glutathione-S-transferase substrates (GST). Earlier, 'cyclic' uncoupling activity was reported for 5-bromo-N-(4-chlorophenyl)-3,4-dinitro-2-thiophenamine (BDCT) in isolated rat liver mitochondria (RLM).
View Article and Find Full Text PDFHeavy metals altered the xenobiotic metabolism of rats by targeting the GST enzyme: An in vitro and in silico study.
Toxicology
December 2024
Department of Biochemistry, University of Allahabad, Prayagraj 211002, India. Electronic address:
Among all the heavy metals, Pb, Cd, and As are the most harmful pollutants in the environment. They reach into the organisms via various levels of food chains i.e.
View Article and Find Full Text PDFMolecular and biochemical characterization of a plant-like iota-class glutathione S-transferase from the halotolerant cyanobacterium Halothece sp. PCC7418.
J Appl Microbiol
September 2024
Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.
Article Synopsis
- Scientists found a special type of enzyme called iota-class GST in a type of bacteria that can survive extreme conditions.
- They studied the genes and discovered that one enzyme (H0729) works really well to break down harmful chemicals better than others.
- This research helps us understand how these bacteria protect themselves from stress, like too much salt, by using their enzymes effectively.
Antioxidative and Antiglycative Stress Activities of Selenoglutathione Diselenide.
Pharmaceuticals (Basel)
August 2024
Institute of Advanced Biosciences, Tokai University, Kitakaname, Hiratsuka 259-1292, Kanagawa, Japan.
The damage caused by oxidative and glycative stress to cells accumulates on a daily basis and accelerates aging. Glutathione (GSH), a major antioxidant molecule in living organisms, plays a crucial role in detoxifying the stress-causing substances inherent in cells, such as HO and methylglyoxal (MG), an important intermediate of advanced glycation end-products (AGEs). In this study, we focused on the enhanced antioxidant capacity of the selenium analog of GSH, i.
View Article and Find Full Text PDFNaphthalene and phenanthrene affect differentially two glutathione S-transferases (GSTs) expression, GST activity, and glutathione content in white shrimp P. vannamei.
Aquat Toxicol
August 2024
Group of Comparative Biochemistry and Physiology. Centro de Investigación en Alimentación y Desarrollo (CIAD), A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo, Sonora, C.P. 83304, Mexico. Electronic address:
Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants ubiquitous in coastal ecosystems. The white shrimp Penaeus vannamei naturally inhabits in coastal areas and is cultivated in farms located nearby the oceans. PAHs can damage shrimp health, endanger natural populations, and lower shrimp aquaculture productivity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!