Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials.

Biomaterials

Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstr. 19, 79104, Germany; Institute for Macromolecular Chemistry, Univ. of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany; Faculty of Chemistry and Pharmacy, Univ. of Freiburg, Fahnenbergplatz, 79085 Freiburg, Germany; Faculty of Biology, Univ. of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany; Institute for Pharmaceutical Sciences, Univ. of Freiburg, Albertstr. 25, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, Univ. of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany. Electronic address:

Published: October 2014

The access to defined protein-based material systems is a major challenge in bionanotechnology and regenerative medicine. Exact control over sequence composition and modification is an important requirement for the intentional design of structure and function. Herein structural- and matrix proteins provide a great potential, but their large repetitive sequences pose a major challenge in their assembly. Here we introduce an integrative "one-vector-toolbox-platform" (OVTP) approach which is fast, efficient and reliable. The OVTP allows for the assembly, multimerization, intentional arrangement and direct translation of defined molecular DNA-tecton libraries, in combination with the selective functionalization of the yielded protein-tecton libraries. The diversity of the generated tectons ranges from elastine-, resilin, silk- to epitope sequence elements. OVTP comprises the expandability of modular biohybrid-materials via the assembly of defined multi-block domain genes and genetically encoded unnatural amino acids (UAA) for site-selective chemical modification. Thus, allowing for the modular combination of the protein-tecton library components and their functional expansion with chemical libraries via UAA functional groups with bioorthogonal reactivity. OVTP enables access to multitudes of defined protein-based biohybrid-materials for self-assembled superstructures such as nanoreactors and nanobiomaterials, e.g. for approaches in biotechnology and individualized regenerative medicine.

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http://dx.doi.org/10.1016/j.biomaterials.2014.06.048DOI Listing

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