Recent in vitro work using purified enzymes demonstrated that nicotine and/or a nicotine metabolite could inhibit CYPs (CYP2A6, 2A13, 2E1) involved in the metabolism of the genotoxic tobacco nitrosamine NNK. This observation raises the possibility of nicotine interaction with the mechanism of NNK bioactivation. Therefore, we hypothesized that nicotine or a nicotine metabolite such as cotinine might contribute to the inhibition of NNK-induced DNA strand breaks by interfering with CYP enzymes. The effect of nicotine and cotinine on DNA strand breaks was evaluated using the COMET assay in CYP competent HepaRG cells incubated with bioactive CYP-dependent NNK and CYP-independent NNKOAc (4-(acetoxymethylnitrosoamino)-1-(3-pyridyl)-1-butanone). We report a dose-dependent reduction in DNA damage in hepatic-derived cell lines in the presence of nicotine and cotinine. Those results are discussed in the context of the in vitro model selected.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tiv.2014.06.017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
National scale assessment of the occurrence and risk of trace organic contaminants in Canadian Lake sediments.
Sci Total Environ
January 2025
Department of Chemistry, Université de Sherbrooke, Sherbrooke, QC, Canada; Université de Sherbrooke Water Research Group (GREAUS), Université de Sherbrooke, Sherbrooke, QC, Canada. Electronic address:
In Canada studies on the presence of trace organic contaminants (TrOCs) such as pharmaceuticals, personal care products, pesticides and flame retardants in lakes have primarily focused on the water column at localized scales. To address this gap, the occurrence of 44 TrOCs, representative of various types of human activities, was investigated in surface sediments (0-2 cm) from 193 lakes across Canada. A total of 28 targeted TrOCs were detected, with 99.
View Article and Find Full Text PDFEfficacy of shared decision making in tobacco cessation among health facilities of Haryana, India - A double blinded, parallel group Randomized Controlled trial Protocol.
Public Health Pract (Oxf)
June 2025
Department of Psychiatry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Background: Shared decision-making (SDM) incorporates evidence, patient values, and preferences into medical decision-making. SDM and decision aids might promote health professional engagement and patient knowledge of tobacco cessation therapy, improving usage and results. The SDM facilitates talks that lead to better-informed judgements that align with patients' priorities, unlike individual decision-making.
View Article and Find Full Text PDFCo-exposure to polycyclic aromatic hydrocarbons and nicotine and their associations with cognitive impairment risk in older adults from southern China.
Food Chem Toxicol
January 2025
School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, China. Electronic address:
Exposure to polycyclic aromatic hydrocarbons (PAHs) and tobacco smoke is widespread and linked to various adverse health outcomes. Their potential to disturb the neurological system has raised much concern, particularly among older adults. Thus, we conducted a case-control study to assess the associations between co-exposure to PAHs and nicotine, and the risk of cognitive impairment and oxidative stress in older adults.
View Article and Find Full Text PDFCotinine, trans-3'-hydroxycotinine, and nicotine metabolite ratio indicate association between smoking and tooth loss.
J Periodontol
January 2025
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Background: Previous research has indicated a potential connection between smoking and tooth loss, but it remains unclear how the metabolites of nicotine, cotinine (COT) and trans-3'-hydroxycotinine (HC), and the nicotine metabolite ratio (NMR) affect the occurrence and progress of tooth loss. In this study, we aimed to investigate the relationship between tooth loss and smoking metabolites, then verify how the systemic immunoinflammatory index (SII) or monocyte to high-density lipoprotein cholesterol ratio (MHR) levels mediate this process.
Methods: The cross-sectional study data were collected from the National Health and Nutrition Examination Survey (NHANES).
Cotinine-measured secondhand smoke exposure among federal housing assistance recipients without active tobacco or nicotine in the United States: NHANES, 2007-2018.
Nicotine Tob Res
January 2025
U.S. Department of Housing and Urban Development, Office of Policy Development and Research, Washington, DC, United States.
Introduction: The prevalence of cigarette smoking is high among U.S. Department of Housing and Urban Development (HUD) assistance recipients, putting others at risk for secondhand smoke (SHS) exposure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!