Long-term performance is of primary concern when considering the commercialized use of an microbial fuel cell(MFC). The long-term stability of MFC was studied using dairy manure as substrate over a testing time of 171 d. The results showed that the MFC could efficiently recover electricity from dairy manure during the long-time run, and the average power density was 6. 77 Wm-3 +/- 2. 11 W m-3. On day 70, the polarization curve was measured, the open circuit voltage, internal resistance and maximum power density of MFC were 0. 874 V, 22. 1 omega and 14. 1 W.m -3, respectively. The 30-day TCOD removal decreased with increasing test time, and the CE during the 121-150 d period reached 17.5% +/-3.3%. Phylogenetic analyses revealed that the dominant microbial communities in anodic biofilm were Proteobacteria (45%) , Bacteroidetes (22%) , Firmicutes (17% ) and Actinobacteria (11% ). At genera level, the bacteria with electricity production and (or) cellulose degradation ability, such as Clostridium and Cellulomonas were the abundant taxa.
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