Antifungal activity of Stenotrophomonas maltophilia in Stomoxys calcitrans larvae.

Rev Bras Parasitol Vet

Departamento de Medicina e Cirurgia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro ? UFRRJ, Seropédica, RJ, Brasil.

Published: June 2016

AI Article Synopsis

  • The microbiota in Stomoxys calcitrans larvae may help them survive in polluted environments by producing substances that inhibit other organisms.
  • Researchers used methods like PCR and scanning electron microscopy to identify a bacterium found in the larvae, which turned out to be Stenotrophomonas maltophilia.
  • This bacterium showed antifungal effects against specific strains of Beauveria bassiana, implying that the larval microbiota could interfere with using this fungus for controlling S. calcitrans larvae.

Article Abstract

The microbiota present in Stomoxys calcitrans larvae may assist their survival in contaminated environments through production of inhibitory substances. Bacteriological identification methods, the polymerase chain reaction (PCR) and scanning electron microscopy (SEM) were used to detect a bacterium naturally present in mucus and macerated S. calcitrans larvae. The antifungal activity was determined based on the results from disk diffusion tests on an artificial solid medium. The bacterium was identified as Stenotrophomonas maltophilia and presented antifungal activity against Beauveria bassiana sensu lato isolates CG 138, CG 228 and ESALQ 986. This result suggests that the larval microbiota is a factor that can compromise the use of B. bassiana s.l. fungus for biological control of S. calcitrans larvae.

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Source
http://dx.doi.org/10.1590/s1984-29612014037DOI Listing

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