AI Article Synopsis
- An enzyme sensor unit was created by encapsulating acetylcholinesterase in a hybrid mesoporous silica membrane, enhancing its stability and functionality.
- The sensor can detect acetylcholine and organophosphorus pesticides like dichlorvos, aldicarb, and parathion with high sensitivity, achieving detection limits equal to or better than existing methods.
- This immobilized enzyme sensor offers advantages such as reusability and prolonged storage stability, making it a practical solution for environmental monitoring.
Article Abstract
An acetylcholinesterase-immobilized sensor unit was successfully prepared by encapsulating the enzyme within hybrid mesoporous silica membranes (F127-MST). Through a novel combination with tetracyanoquinodimethane, both acetylcholine and organophosphorus pesticides were successfully detected with high sensitivity. Furthermore, we manufactured the working prototype of an enzyme sensor with this sensor unit for detecting dichlorvos, aldicarb and parathion. At present, the detection limit in this working prototype either equaled or surpassed that of others. Also, we have the advantage of increased stability of the enzyme against the outer environment by encapsulation of the enzymes into a silica nanospace. Consequently, acetylcholinesterase immobilized in F127-MST is a practical sensor with high sensitivity, reusability, and storage stability.
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| http://dx.doi.org/10.1039/c4an00975d | DOI Listing |
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