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CLADES: A Programmable Cascade of Genes for Cell Lineage Analysis and Manipulation.
Methods Mol Biol
January 2025
Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
In the Drosophila brain, neuronal diversity originates from approximately 100 neural stem cells, each dividing asymmetrically. Precise mapping of cell lineages at the single-cell resolution is crucial for understanding the mechanisms that direct neuronal specification. However, existing methods for high-resolution lineage tracing are notably time-consuming and labor-intensive.
View Article and Find Full Text PDFBullet characterization using Photon-Counting detector CT: A phantom study with intact bullets.
Eur J Radiol
December 2024
Department of Radiology and Nuclear Medicine, Maastricht University Medical Centre+, PO Box 5800, 6202 AZ Maastricht, the Netherlands; Mental Health and Sciences (MHeNs) Research Institute, Maastricht University, PO Box 616, 6200 MD Maastricht, the Netherlands.
Objectives: Photon-counting detector CT (PCD-CT) is expected to substantially improve and expand CT-imaging applicability due to its intrinsic spectral capabilities, increased spatial resolution, reduced electronic noise, and improved image contrast. The current study aim is to evaluate PCD-CT efficacy in characterizing bullets based on their dimensions, shape, and material composition.
Materials And Methods: This is an observational phantom study examining 11 unfired, intact bullets of various common calibers, placed in ballistic gelatin.
Cholera, an acute waterborne diarrheal disease, remains a major global health challenge. Despite being curable and preventable, it can be fatal if left untreated, especially for children. Bangladesh, a cholera-endemic country with a high disease burden, experiences two peaks annually, during the dry pre-monsoon spring and the wet post-monsoon fall seasons.
View Article and Find Full Text PDFAggregation-induced color-coded imaging for HOCl detection in living cells with dark-field microscopy.
Talanta
February 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China. Electronic address:
In this work, we develop a nanoprobe for the detection of hypochlorous acid (HOCl), a key reactive oxygen species, utilizing dark-field microscopy (DFM). The nanoprobe is constructed by covalently attaching PEG-SH and an HOCl-sensitive molecule, FD, to gold nanoparticles (GNPs-FD-PEG). This probe detects HOCl by observing a color shift from green to red due to the aggregation of GNPs, which is triggered by HOCl-induced the cleavage of FD molecule.
View Article and Find Full Text PDFB-Lines in the Assessment of Interstitial Lung Disease Associated with Scleroderma: The Role of Handheld Devices.
Diagnostics (Basel)
October 2024
Internal Medicine Department, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
: Timely detection and aggressive management of interstitial lung disease (ILD) in systemic sclerosis (SSc) are essential to improving outcomes and reducing risks of irreversible lung injury. : to explore the usefulness of an ultraportable ultrasound device for the management of SSc-related ILD and to compare it with clinical and instrumental data. : A total of 19 consecutive SSc patients underwent a comprehensive pulmonary evaluation: clinical, pulmonary function tests (PFTs) (spirometry, DLCO), lung CT (1.
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