AI Article Synopsis
- Researchers sequenced internal transcribed spacer regions of ribosomal DNA and created species-specific primers to differentiate between two similar mosquito species, Ochlerotatus detritus and Ochlerotatus coluzzii.
- The primers effectively produced unique fragments of varying sizes, enabling successful identification of samples from France and Germany.
- The findings support further ecological research to improve understanding and management of these mosquito species, while also identifying distinct haplotypes present in different regions, including a unique group in mosquitoes from Tunisia, raising questions about their classification.
Article Abstract
Internal transcribed spacer regions of ribosomal DNA were sequenced, and species-specific primers were designed to simplify the identification of two morphologically similar species of the Detritus complex, Ochlerotatus detritus and Ochlerotatuscoluzzii. Each newly designed primer was able to amplify a species-specific fragment with a different size. Samples from France and Germany were successfully tested. This new tool prompts for bio-ecological studies to refine basic knowledge on the bionomics of this species complex, towards a better control and prevention of ensuing nuisances. Moreover, ITS2 sequencing revealed the existence of (1) two distinct haplotypes of Oc. detritus that are sympatric and widely distributed along the French Atlantic and Mediterranean littorals and (2) a specific haplotype in mosquitoes sampled from Tunisia, raising the question of the taxonomic status of this North-African population.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.meegid.2014.07.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Using a pupal exuvia to designate the undamaged neotype of a species belonging to a complex of sibling species - the case of Aedes coluzzii (Diptera, Culicidae).
Parasite
March 2022
MIVEGEC, Univ. Montpellier, CNRS, IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
Article Synopsis
- Aedes (Ochlerotatus) coluzzii, a mosquito species, was previously identified from its sibling species Aedes detritus using a destruction method, which left no holotype.
- Researchers aimed to establish a neotype by collecting specimens from the original location and rearing them until adulthood.
- They successfully extracted genomic DNA from pupal exuvia to verify the species identity and designated a male as the neotype, marking a significant advancement in mosquito taxonomy through molecular identification.
Laboratory transmission potential of British mosquitoes for equine arboviruses.
Parasit Vectors
August 2020
Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.
Background: There has been no evidence of transmission of mosquito-borne arboviruses of equine or human health concern to date in the UK. However, in recent years there have been a number of outbreaks of viral diseases spread by vectors in Europe. These events, in conjunction with increasing rates of globalisation and climate change, have led to concern over the future risk of mosquito-borne viral disease outbreaks in northern Europe and have highlighted the importance of being prepared for potential disease outbreaks.
View Article and Find Full Text PDFPotential for Zika virus transmission by mosquitoes in temperate climates.
Proc Biol Sci
July 2020
Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park-Innovation Centre 2, 131 Mount Pleasant, Liverpool L3 5TF, UK.
Mosquito-borne Zika virus (ZIKV) transmission has almost exclusively been detected in the tropics despite the distributions of its primary vectors extending farther into temperate regions. Therefore, it is unknown whether ZIKV's range has reached a temperature-dependent limit, or if it can spread into temperate climates. Using field-collected mosquitoes for biological relevance, we found that two common temperate mosquito species, and , were competent for ZIKV.
View Article and Find Full Text PDFA Survey of Aedes (Diptera: Culicidae) Mosquitoes in Tunisia and the Potential Role of Aedes detritus and Aedes caspius in the Transmission of Zika Virus.
J Med Entomol
September 2019
Laboratory of Epidemiology and Veterinarian Microbiology, Medical Entomology, Institut Pasteur, 13 Place Pasteur, B.P. 74., 1002 Tunis-Belvédère, Tunisia.
The present study aimed to update the list of Aedes mosquito species occurring in Tunisia and to test the vector competence of Aedes (Ochlerotatus) caspius (Pallas) and Ae. (Ochlerotatus) detritus (Haliday), the locally most abundant and widespread species, to transmit Zika virus (ZIKV). In 2017-2018, mosquito larvae were collected from 39 different larval habitats in seven bioclimatic zones of Tunisia.
View Article and Find Full Text PDFConditioning of Leaf Detritus Modulates Density-Dependent Growth of Aedes triseriatus Larvae (Diptera: Culicidae).
J Med Entomol
February 2018
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI.
Ration of food per capita of larval population is widely thought to limit mosquito production from container habitats, directly reflecting resource limitation. In this study, the importance of density-dependent resource limitation on larval Aedes triseriatus (Say) (Diptera: Culicidae) depended on the degree of microbial conditioning of senescent leaf detritus in surrogate tree holes. Density and ration strongly affected emergence, total female mass, and mean female mass of populations grown on leaves that had conditioned for 3 d, but had less impact when larval populations were grown on leaves that had conditioned for 1 mo.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!