AI Article Synopsis
- A plasmid was created to express FLO11, a gene that codes for a glycoprotein in yeast, S. cerevisiae, using a constant promoter.
- This plasmid allowed a non-pellicle-forming industrial strain of the yeast to form a pellicle (a surface film) in a glucose-based liquid medium.
- The cells with the new capability showed increased resistance to furfural, a toxic compound that can hinder ethanol production from plant materials.
Article Abstract
We constructed a plasmid that expresses FLO11 encoding a cell surface glycoprotein of Saccharomyces cerevisiae under the control of a constitutive promoter. This plasmid conferred pellicle-forming ability on the non-pellicle-forming industrial strain of S. cerevisiae at the air-liquid interface of the glucose-containing liquid medium. The induced pellicle-forming cells exhibited tolerance to furfural, which is a key toxin in lignocellulosic hydrolysates, in ethanol production.
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| http://dx.doi.org/10.1080/09168451.2014.895660 | DOI Listing |
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