Antisense RNA (asRNA) COOLAIR is expressed at A. thaliana FLOWERING LOCUS C (FLC) in response to winter temperatures. Its contribution to cold-induced silencing of FLC was proposed but its functional and evolutionary significance remain unclear. Here we identify a highly conserved block containing the COOLAIR first exon and core promoter at the 3' end of several FLC orthologues. Furthermore, asRNAs related to COOLAIR are expressed at FLC loci in the perennials A. alpina and A. lyrata, although some splicing variants differ from A. thaliana. Study of the A. alpina orthologue, PERPETUAL FLOWERING 1 (PEP1), demonstrates that AaCOOLAIR is induced each winter of the perennial life cycle. Introduction of PEP1 into A. thaliana reveals that AaCOOLAIR cis-elements confer cold-inducibility in this heterologous species while the difference between PEP1 and FLC mRNA patterns depends on both cis-elements and species-specific trans-acting factors. Thus, expression of COOLAIR is highly conserved, supporting its importance in FLC regulation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109010 | PMC |
| http://dx.doi.org/10.1038/ncomms5457 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessing Genetic Diversity and Population Structure of Western Honey Bees in the Czech Republic Using 22 Microsatellite Loci.
Insects
January 2025
Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
To date, no study has been conducted to investigate the diversity in honeybee populations of in the Czech Republic. Between 2022 and 2023, worker bees were collected from colonies distributed throughout the Czech Republic in 77 districts, and their genetic differences were examined using 22 microsatellite loci. The samples were obtained from hives ( = 3647) and through the process of capture on flowers ( = 553).
View Article and Find Full Text PDFAnalysis of the genetic basis of fiber-related traits and flowering time in upland cotton using machine learning.
Theor Appl Genet
January 2025
State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
Cotton is an important crop for fiber production, but the genetic basis underlying key agronomic traits, such as fiber quality and flowering days, remains complex. While machine learning (ML) has shown great potential in uncovering the genetic architecture of complex traits in other crops, its application in cotton has been limited. Here, we applied five machine learning models-AdaBoost, Gradient Boosting Regressor, LightGBM, Random Forest, and XGBoost-to identify loci associated with fiber quality and flowering days in cotton.
View Article and Find Full Text PDFGenetic dissection of foxtail millet bristles using combined QTL mapping and RNA-seq.
Theor Appl Genet
January 2025
College of Agriculture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, China.
QTL mapping of two RIL populations in multiple environments revealed a consistent QTL for bristle length, and combined with RNA-seq, a potential candidate gene influencing bristle length was identified. Foxtail millet bristles play a vital role in increasing yields and preventing bird damage. However, there is currently limited research on the molecular regulatory mechanisms underlying foxtail millet bristle formation, which constrains the genetic improvement and breeding of new foxtail millet varieties.
View Article and Find Full Text PDFSmall molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis.
Commun Biol
January 2025
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology (NAIST), Ikoma, Japan.
Monocarpic plants flower only once and then produce seeds. Many monocarpic plants require a cold treatment known as vernalization before they flower. This requirement delays flowering until the plant senses warm temperatures in the spring.
View Article and Find Full Text PDFBRASSINAZOLE RESISTANT 1 delays photoperiodic flowering by repressing CONSTANS transcription.
Plant Physiol
January 2025
Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, and College of Life Sciences, Capital Normal University, Beijing 100048, China.
Photoperiodic regulation of flowering time plays a critical role in plant reproductive success and crop yield. In Arabidopsis thaliana, the expression of the CONSTANS (CO) gene is closely regulated by day length and is modulated by both environmental and endogenous cues for precise control over flowering. Our findings reveal that the phytohormone brassinosteroid (BR) pathway represses flowering by inhibiting the expression of both CO and Flowering Locus T (FT).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!