The lysophosphatidic acid receptor 1 (LPA1), a G-protein coupled receptor, regulates cell proliferation, migration, and cytokine release. Here, we investigate the molecular signature of LPA1 trafficking to the cell surface. The overexpressed LPA1 with a C-terminal V5 tag (LPA1-V5) is majorly expressed on the cell surface, while two deletion mutants (C320 and ∆84-87) failed to be trafficked to the cell surface. Further, site-directed mutagenesis analysis of the LPA1 revealed that Ile325, Tyr85, and Leu87 within these two fragments regulate LPA1 maturation and trafficking to the cell surface. Over-expression of Sar1, a component of coat protein complex II (COPII), enhances glycosylation of LPA1 wild type, but not these mutants. The mutants of LPA1 are majorly localized in the endoplasmic reticulum (ER) and exhibit a higher binding affinity to heat shock protein 70 (Hsp70), when compared to the LPA1 wild type. Further, we found that all these mutants failed to increase phosphorylation of Erk, and the cytokine release in response to LPA treatment. These results suggest that Ile325, Tyr85, and Leu87 within LPA1 are essential for LPA1 protein properly folding in the ER.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225160 | PMC |
| http://dx.doi.org/10.1016/j.cellsig.2014.07.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A viscoelastic-plastic deformation model of hemisphere-like tip growth in Arabidopsis zygotes.
Quant Plant Biol
December 2024
Department of Mechanical Engineering, Faculty of Systems Science and Technology, Akita Prefectural University, Yurihonjo, Japan.
Plant zygote cells exhibit tip growth, producing a hemisphere-like tip. To understand how this hemisphere-like tip shape is formed, we revisited a viscoelastic-plastic deformation model that enabled us to simultaneously evaluate the shape, stress and strain of Arabidopsis () zygote cells undergoing tip growth. Altering the spatial distribution of cell wall extensibility revealed that cosine-type distribution and growth in a normal direction to the surface create a stable hemisphere-like tip shape.
View Article and Find Full Text PDFFeMOFs/CO loading reduces NETosis and macrophage inflammatory response in PLA based cardiovascular stent materials.
Regen Biomater
December 2024
Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
Modification of polylactic acid (PLA) is a promising strategy for the next generation of bioresorbable vascular stent biomaterials. With this focus, FeMOFs nanoparticles was incorporated in PLA, and then post loading of carbon monoxide (CO) was performed by pressurization. It showed FeMOFs incorporation increased hydrophilicity of the surface and CO loading, and CO release was sustained at least for 3 days.
View Article and Find Full Text PDFProgress in understanding the regulatory mechanisms of immune checkpoint proteins PD-1 and PD-L1 expression.
Clin Transl Oncol
January 2025
Center of Translational Medicine, Zibo Central Hospital, Shandong Second Medical University, 54 Gongqingtuan Xi Road, Zibo, 255036, Shandong, China.
Programmed Death Protein-1 (PD-1) is a cell surface receptor that serves as a checkpoint for T cells, playing a pivotal role in regulating T-cell apoptosis. The binding of PD-1 to its ligand, Programmed Death Ligand 1 (PD-L1), inhibits anti-tumor immunity by suppressing T-cell activation signals. Indeed, the PD-1/PD-L1 pathway governs the induction and maintenance of immune tolerance within the tumor microenvironment.
View Article and Find Full Text PDFAnchoring Sn Nanoparticles in Necklace-Like B,N,F-Doped Carbon Fibers Enables Anode-Less 5V-Class Li-Metal Batteries.
Angew Chem Int Ed Engl
January 2025
City University of Hong Kong, Department of Chemistry, 83 Tat Chee Avenue, Hong Kong, HONG KONG.
Li metal batteries (LMBs), particularly with a limited Li metal anode and a 5V-class cathode, offer significantly higher energy density compared to the state-of-the-art Li-ion batteries. However, the limited Li anode poses severe challenges to cycling stability due to low efficiency and large volume expansion issues associated with Li. Herein, we design a lightweight and functionalized host composed of Sn nanoparticles embedded into necklace-like B,N,F-doped carbon macroporous fibers (Sn@B/N/F-CMFs) toward anode-less 5V-class LMBs.
View Article and Find Full Text PDFEnhancing CO2 Electroreduction to Multicarbon Products by Modulating the Surface Microenvironment of Electrode with Polyethylene Glycol.
Angew Chem Int Ed Engl
January 2025
Institute of Chemistry Chinese Academy of Sciences, Institute of chemistry, Beiyijie number 2, Zhongguancun, 100190, Beijing, CHINA.
Modulating the surface microenvironment of electrodes stands as a pivotal aspect in enhancing the electrocatalytic performance for CO2 electroreduction. Herein, we propose an innovative approach by incorporating a small amount of linear oligomer, polyethylene glycol (PEG), into Cu2O catalysts during the preparation of the CuPEG electrode. The Faradaic efficiency (FE) toward multicarbon products (C2+) increases from 69.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!