AI Article Synopsis
Article Abstract
Objective: This study aimed to assess the overall apatite crystals profile in the enamel matrix of mice susceptible (A/J strain) or resistant (129P3/J strain) to dental fluorosis through analyses by atomic force microscopy (AFM).
Material And Methods: Samples from the enamel matrix in the early stages of secretion and maturation were obtained from the incisors of mice from both strains. All detectable traces of matrix protein were removed from the samples by a sequential extraction procedure. The purified crystals (n=13 per strain) were analyzed qualitatively in the AFM. Surface roughness profile (Ra) was measured.
Results: The mean (±SD) Ra of the crystals of A/J strain (0.58±0.15 nm) was lower than the one found for the 129P3/J strain (0.66±0.21 nm) but the difference did not reach statistical significance (t=1.187, p=0.247). Crystals of the 129P3/J strain (70.42±6.79 nm) were found to be significantly narrower (t=4.013, p=0.0013) than the same parameter measured for the A/J strain (90.42±15.86 nm).
Conclusion: enamel crystals of the 129P3/J strain are narrower, which is indicative of slower crystal growth and could interfere in the occurrence of dental fluorosis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072265 | PMC |
| http://dx.doi.org/10.1590/1678-775720130515 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Variation in the gene reveals complex temporal properties of mouse brainstem taste responses to sweeteners.
Am J Physiol Regul Integr Comp Physiol
November 2021
Center for Medical Education, Ball State University, Muncie, Indiana.
The gene codes for the protein T1R3, which dimerizes with T1R2 to form a sweetener-binding receptor in taste cells. influences sweetener preferences in mice, as shown by work with a 129.B6-Tas1r3 segregating congenic strain on a 129P3/J (129) genetic background; members of this strain vary in whether they do or do not have one copy of a donor fragment with the C57BL/6ByJ (B6) allele for (B6/129 and 129/129 mice, respectively).
View Article and Find Full Text PDFGenetics of mouse behavioral and peripheral neural responses to sucrose.
Mamm Genome
April 2021
Monell Chemical Senses Center, Philadelphia, PA, USA.
Mice of the C57BL/6ByJ (B6) strain have higher consumption of sucrose, and stronger peripheral neural responses to it, than do mice of the 129P3/J (129) strain. To identify quantitative trait loci (QTLs) responsible for this strain difference and to evaluate the contribution of peripheral taste responsiveness to individual differences in sucrose intake, we produced an intercross (F) of 627 mice, measured their sucrose consumption in two-bottle choice tests, recorded the electrophysiological activity of the chorda tympani nerve elicited by sucrose in a subset of F mice, and genotyped the mice with DNA markers distributed in every mouse chromosome. We confirmed a sucrose consumption QTL (Scon2, or Sac) on mouse chromosome (Chr) 4, harboring the Tas1r3 gene, which encodes the sweet taste receptor subunit TAS1R3 and affects both behavioral and neural responses to sucrose.
View Article and Find Full Text PDFGenetic controls of Tas1r3-independent sucrose consumption in mice.
Mamm Genome
April 2021
Monell Chemical Senses Center, Philadelphia, PA, USA.
Article Synopsis
- Researchers mapped a quantitative trait locus (QTL) on mouse chromosome 4, which influences sucrose consumption, called Scon2, linked to the Tas1r3 gene responsible for sweet taste receptors.
- In an effort to find additional sucrose consumption QTLs, scientists created hybrids that excluded Scon2 and identified two main QTLs, Scon3 on chromosome 9 and Scon10 on chromosome 14, as well as an interacting pair, Scon3 and Scon4.
- Their studies indicated that Scon3 and Scon4 affect sucrose intake not through taste but rather through energy metabolism, with specific genotype influences leading to variations in sucrose drinking behavior and energy expenditure in mice.
Who is HOT and who is LOT? Detailed characterization of prescription opioid-induced changes in behavior between 129P3/J and 129S1/SvlmJ mouse substrains.
Genes Brain Behav
June 2020
Department of Molecular, Developmental and Cell Biology, University of California Santa Barbara, Santa Barbara, California.
Genetic factors are theorized to contribute to the substantial inter-individual variability in opioid abuse/addiction. To advance the behavioral genetics of prescription opioid abuse, our prior work identified the 129S1/SvlmJ (S1) and related 129P3/J (P3) mouse substrains, respectively, as low and high opioid-taking. Herein, we related our prior results to measures of sucrose reward/reinforcement, basal anxiety, opioid-induced place-conditioning, locomotor activity and Straub tail reaction, as well as behavioral and physiological signs of withdrawal.
View Article and Find Full Text PDFAnalysis of Polymorphisms in Genes Differentially Expressed in the Enamel of Mice with Different Genetic Susceptibilities to Dental Fluorosis.
Caries Res
December 2019
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru,
Genes expressed during amelogenesis are candidates to increase the risk of dental fluorosis (DF). Thus, this study aimed to evaluate the association between polymorphisms in enamel development genes and susceptibility to DF in mice. Mice of both sexes, representing strains 129P3/J (n = 20; resistant to DF) and A/J (n = 20; susceptible to DF), were divided into 2 groups.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!