A simple technique for the identification of common genotypes of the hepatitis B virus (HBV) remains to be identified. The present study was conducted to establish such a methodology. Four plasmids of genotypes A‑D and 123 clinical serum specimens of HBV‑infected patients were genotyped. HBV genotypes would be detected successfully when the HBV genotype reached a viral load of 1 x 103 copies/ml or the BC genotype mixed samples reached a 5% level. The lower limit of detection of HBV DNA in serum specimens was determined to be 2.14x102 IU/ml. The assay sensitivity and specificity were 100% and the consistency was demonstrated to reach as high as 90.24 and 100% compared with that of the DNA sequencing and cloning. The frequencies of the genotypes B, C, BC, BD and BCD were found to be 65.0, 23.6, 7.3, 3.3 and 0.8%, respectively. The accuracy of detection of the mixed infections was also higher using the rapid and simple SNaPshot method compared with that achieved with the DNA sequencing methods. The results of the present study indicated that the SNaPshot technique accurately distinguishes the HBV genotypes A‑D and is able to be readily applied as a monitoring tool in HBV prognosis and treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121402 | PMC |
| http://dx.doi.org/10.3892/mmr.2014.2372 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and characterization of gold nanoparticle-based colorimetric sensing assay functionalized with L-cysteine for the detection of calcium carbide in ripened mango and banana fruits.
Heliyon
February 2025
Department of Nanotechnology, North-Eastern Hill University (NEHU), East Khasi Hills, Shillong, 793022, Meghalaya, India.
Calcium carbide (CaC) is generally used as an artificial ripening agent in the agricultural and food sectors but has been prohibited due to its negative impacts on the environment and human. Therefore, in the present study, a novel and cost-effective detection technique was developed for the detection of CaC using L-cysteine functionalized gold nanoparticles (AuNPs). In this work, a rapid, simple, environment friendly and portable colorimetric nanosensing assay was developed using two different sizes of L-cysteine functionalized gold nanoparticles (AuNP and AuNP).
View Article and Find Full Text PDFStudy on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices.
Biomed Chromatogr
April 2025
Ankang Inspection and Testing Center for Food and Drug, Ankang, China.
Topical poultices and applied medical devices that can relieve a variety of cervical vertebrae and lumbar vertebrae pain may have illegal additives of pharmaceutical drugs or analogues have additional health risks, but customers may not be aware of what they are using. National Medical Products Administration reviewed and approved "Supplementary test method for identification and content determination of 17 chemicals in affixed medical devices" drafted by Shandong Provincial Institute of Medical Devices and Drug Packaging Inspection in January 2022, but the test method is simple in sample processing and difficult to extract the chemical components in the paste that may cause the phenomenon of missing detection of most illegal added chemical drugs. In this work, the sample handling and extraction methods have been improved and optimized, the content of illegal added chemicals in 47 batches of affixed medical devices was determined by HPLC-MS/MS method and was detected in 37 batches, and the non-steroidal anti-inflammatory drug nimesulide and aspirin were detected in most samples.
View Article and Find Full Text PDFEstablishment and validation of a simple and accurate qPCR detection method for Haemophilus parasuis.
Sci Rep
March 2025
Department of Neurology, China-Japan Union Hospital of Jilin University, 130033, Changchun, Jilin, China.
As an infectious disease that poses a significant threat to the rapidly growing pig breeding industry, the detection of Haemophilus parasuis (HPS) is often compromised by various interfering substances present in the test sample during quantitative real-time PCR (qPCR). The rapid detection of HPS is important for the isolation of infectious pigs and their treatment. We designed and optimized a rapid qPCR test to detect the INFB gene of HPS in clinical and environmental samples on pig farms.
View Article and Find Full Text PDFCRISPR-Cas-Based Diagnosis of Geminiviruses.
Methods Mol Biol
March 2025
Amity Institute of Virology & Immunology, Amity University Uttar Pradesh, Noida, India.
Successful disease management relies on rapid and accurate identification of the causal agent. Begomoviruses (family Geminiviridae) cause severe economic losses and pose a serious threat to sustainable agriculture. Here, we describe a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection method utilizing the CRISPR/Cas12a and CRISPR/Cas13a system for begomoviruses used in our laboratory.
View Article and Find Full Text PDFSAMP-Net: a medical image segmentation network with split attention and multi-layer perceptron.
Med Biol Eng Comput
March 2025
School of Electrical and Information Engineering, Beijing University of Civil Engineering and Architecture, No.15, Yongyuan Road, Huangcun Town, Daxing District, Beijing, 102616, China.
Convolutional neural networks (CNNs) have achieved remarkable success in computer vision, particularly in medical image segmentation. U-Net, a prominent architecture, marked a major breakthrough and remains widely used in practice. However, its uniform downsampling strategy and simple stacking of convolutional layers in the encoder limit its ability to capture rich features at multiple depths, reducing its efficiency for rapid image processing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!