AI Article Synopsis
- The study focuses on the genus Ocimum, known for its medicinal and aromatic properties, by performing transcriptome sequencing on two key species, O. sanctum and O. basilicum, to understand their genomic resources.
- The sequencing resulted in over 69,000 transcripts for O. sanctum and over 130,000 for O. basilicum, with a strong amount of those being related to secondary metabolism pathways, particularly in terpenoid and phenylpropanoid biosynthesis.
- This research provides a foundational comparative analysis of Ocimum species, offering insights that could aid in gene characterization and the development of plants with desirable essential oil profiles.
Article Abstract
Background: Ocimum L. of family Lamiaceae is a well known genus for its ethnobotanical, medicinal and aromatic properties, which are attributed to innumerable phenylpropanoid and terpenoid compounds produced by the plant. To enrich genomic resources for understanding various pathways, de novo transcriptome sequencing of two important species, O. sanctum and O. basilicum, was carried out by Illumina paired-end sequencing.
Results: The sequence assembly resulted in 69117 and 130043 transcripts with an average length of 1646 ± 1210.1 bp and 1363 ± 1139.3 bp for O. sanctum and O. basilicum, respectively. Out of the total transcripts, 59648 (86.30%) and 105470 (81.10%) from O. sanctum and O. basilicum, and respectively were annotated by uniprot blastx against Arabidopsis, rice and lamiaceae. KEGG analysis identified 501 and 952 transcripts from O. sanctum and O. basilicum, respectively, related to secondary metabolism with higher percentage of transcripts for biosynthesis of terpenoids in O. sanctum and phenylpropanoids in O. basilicum. Higher digital gene expression in O. basilicum was validated through qPCR and correlated to higher essential oil content and chromosome number (O. sanctum, 2n = 16; and O. basilicum, 2n = 48). Several CYP450 (26) and TF (40) families were identified having probable roles in primary and secondary metabolism. Also SSR and SNP markers were identified in the transcriptomes of both species with many SSRs linked to phenylpropanoid and terpenoid pathway genes.
Conclusion: This is the first report of a comparative transcriptome analysis of Ocimum species and can be utilized to characterize genes related to secondary metabolism, their regulation, and breeding special chemotypes with unique essential oil composition in Ocimum.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125705 | PMC |
| http://dx.doi.org/10.1186/1471-2164-15-588 | DOI Listing |
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