AI Article Synopsis
- Immunofluorescence is a lab technique that uses antibodies tagged with fluorescent markers to identify immune complexes in tissues.
- Most clinical antibodies target common domains in human antibodies, enabling the detection of various autoimmune complexes.
- Successful use of this technique requires careful specimen handling and knowledge of fluorophores and antibodies to ensure strong results and troubleshoot any issues.
Article Abstract
Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes. Drawbacks to this technique mostly relate to proper handling of the specimen and the fluorophore-labeled antibodies. Therefore, having a basic understanding of fluorophores and antibodies is important for processing a specimen that yields a high signal-to-background ratio as well as troubleshooting problems, should they arise.
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| http://dx.doi.org/10.1007/978-1-4939-1050-2_6 | DOI Listing |
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