Background And Aims: Leukocyte-derived proteins in faeces, especially calprotectin, are increasingly used to assess disease activity in ulcerative colitis. The objectives of the present study were to assess the importance of factors related to the stool sampling procedure.
Methods: For 2 days, patients with active ulcerative colitis collected two stool samples at each bowel movement. The time of defecation, consistency and presence of blood were self-recorded in a diary. The variability in the concentrations of calprotectin during the day and between two consecutive days was assessed, as was the stability of calprotectin concentrations in samples stored at room temperature.
Results: Altogether, 18 patients collected 287 stool samples. The intraclass correlation coefficient in pairs of samples from 132 bowel movements was 0.79 (95% CI 0.48-0.90). The median individual coefficient of variation in samples collected during the same day was 52% (4-178). There was a correlation between the level of calprotectin and the time between bowel movements (r = 0.5; p = 0.013). After 3 days at room temperature the calprotectin concentrations in stool samples were unchanged, but after 7 days a significant (p < 0.01) decrease was found (mean 28%; 95% CI 0.10-0.47).
Conclusion: The present data reveal a great variability in the concentrations of calprotectin in stool samples collected during a single day. Since the levels of calprotectin increased with longer time between the bowel movements, it seems most appropriate to analyse stool from the first bowel movement in the morning. Moreover, storage of stool samples at room temperature for more than 3 days is not advisable.
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http://dx.doi.org/10.1016/j.crohns.2014.06.002 | DOI Listing |
Pol J Vet Sci
December 2024
University of Warmia and Mazury in Olsztyn, Faculty of Veterinary Medicine, Department of Parasitology and Invasive Diseases, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Birds of prey raised in captivity have direct contact with the environment and are fed raw meat various animals, which increases the risk of infections caused by parasites, including endoparasites. The aim of this study was to evaluate the prevalence of endoparasites in predatory birds of the orders Accipitriformes and Falconiformes that are used in falconry in Poland. Fresh feces were sampled from 52 birds, including 16 saker falcons (Falco cherrug), 8 lanner falcons (Falco biarmicus), 7 peregrine falcons (Falco peregrinus), 8 Harris's hawks (Parabuteo unicinctus), 7 Eurasian goshawks (Accipiter gentilis), 3 common kestrels (Falco tinnunculus), 1 Eurasian sparrowhawk (Accipiter nisus), 1 red-tailed hawk (Buteo jamaicensis), and 1 common buzzard (Buteo buteo).
View Article and Find Full Text PDFIran J Parasitol
January 2024
Department of Invertebrates Zoology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México.
Background: We aimed to develop a sandwich ELISA, using polyclonal antibodies against excretory/secretory (E/S) antigens specific to coproantigens present in -positive dogs.
Methods: Antibodies were produced at Biological Sciences School, Autonomous University of Nuevo León, in 2023 by immunization of rabbits with antigenic extracts from in vitro cultures of larvae. Assays were performed on 100 stool samples from pet dogs, measuring sensitivity, specificity, and cross-reactivity against other parasitic infections.
Iran J Parasitol
January 2024
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Background: is one of the neglected tropical diseases. We aimed to verify the genetic diversity of with attention to clinical features of the infection in patients using the 1 gene and DNA sequencing.
Methods: Using parasitological methods, was isolated from stool samples of patients who had been referred to Tehran University of Medical Sciences, Tehran, Iran.
Front Cell Infect Microbiol
December 2024
Neonatology Department, Affiliated Shenzhen Children's Hospital of Shantou University Medical College, Shenzhen, China.
Background: Women with vulvovaginal candidiasis (VVC) are known to experience vaginal microbial dysbiosis. However, the dynamic alterations of the vaginal microbiome in pregnant women with VVC and its effect on neonatal gut microbiome remain unclear. This study aims to characterize the vaginal microbiome in pregnant women with VVC and its impact on their offspring's meconium microbiome.
View Article and Find Full Text PDFJ Water Health
December 2024
Centro de Vigilancia de Aguas Residuales, Centinela Biobío, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile; Laboratorio de Investigación en Ciencias Biomédicas, Departamento de Ciencias Básicas y Morfología, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile E-mail:
Studies have shown the presence of SARS-CoV-2 in the stool of both symptomatic and asymptomatic COVID-19 patients, enabling wastewater-based surveillance (WBS) to complement clinical monitoring. The emergence of variants can enhance viral transmissibility, highlighting the need for ongoing surveillance to detect and control infectious diseases. This study aimed to detect SARS-CoV-2 variants in wastewater from a treatment plant in San Pedro de la Paz, Chile, between January and November 2021.
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