AI Article Synopsis
- A new non-fluorescent quencher using thiazole orange was added to oligonucleotides to enhance their performance in experiments.
- It was shown to effectively work with fluorescein amidite dyes in fluorimetry and real-time polymerase chain reaction (PCR) tests.
- The thiazole orange quencher improves the stability of DNA duplexes, which can help create shorter and more precise probes, and it was also effective in TaqMan probes.
Article Abstract
A non-fluorescent quencher based on thiazole orange was incorporated into oligonucleotides. Fluorimetry and fluorogenic real-time polymerase chain reaction experiments demonstrated that the quencher is effective for fluorescein amidite dyes. The thiazole orange quencher also increased the melting temperature of DNA duplexes, which may facilitate the design of shorter and more discriminatory probes. The effectiveness of the quencher in TaqMan probes was also demonstrated.
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| http://dx.doi.org/10.1016/j.bmcl.2014.06.036 | DOI Listing |
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