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http://dx.doi.org/10.1038/scientificamerican0614-25 | DOI Listing |
Front Bioeng Biotechnol
February 2023
Institut Curie, Université PSL, Sorbonne Université, CNRS UMR168, Laboratoire Physico Chimie Curie, Paris, France.
Optogenetics arises as a valuable tool to precisely control genetic circuits in microbial cell factories. Light control holds the promise of optimizing bioproduction methods and maximizing yields, but its implementation at different steps of the strain development process and at different culture scales remains challenging. In this study, we aim to control beta-carotene bioproduction using optogenetics in and investigate how its performance translates across culture scales.
View Article and Find Full Text PDFBio Protoc
November 2020
Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Ireland.
This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (, bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person. It provides a platform for parallel, long-term studies of evolution and adaptation of microorganisms under the stress of antimicrobial agents and/or toxic pollutants.
View Article and Find Full Text PDFFront Bioeng Biotechnol
March 2020
Department of Biotechnology, Delft University of Technology, Delft, Netherlands.
The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits deployable at kitchen table. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from transgenic systems has focused on microbial inactivation. However, sterilization methods have not been assessed for DNA release and integrity.
View Article and Find Full Text PDFLab Chip
March 2020
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA. and Department of Electrical and Computer Engineering, University of Connecticut, Storrs, CT 06269, USA.
We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning the unknown diffuser to different x-y positions, we acquire a sequence of modulated intensity images for quantitative object recovery.
View Article and Find Full Text PDFNat Biotechnol
August 2018
Biological Design Center, Boston University, Boston, Massachusetts, USA.
Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell-culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. We report the design and validation of eVOLVER, a scalable do-it-yourself (DIY) framework, which can be configured to carry out high-throughput growth experiments in molecular evolution, systems biology, and microbiology.
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