Pharmacogn Mag
Faculty of Applied Sciences, UCSI University, 56000 Cheras, Kuala Lumpur, Malaysia.
Published: April 2014
Background: Our earlier study on the antiproliferative (APF) activity of leaf extracts of ten Apocynaceae species showed that leaves of Vallaris glabra possessed strong and broad-spectrum properties.
Materials And Methods: In this study, sequential extracts of leaves, flowers and stems, and fractions and isolated compounds from dichloromethane (DCM) leaf extract of V. glabra were assessed for APF activity using the sulphorhodamine B (SRB) assay. Apoptotic effect of MDA-MB-231 cancer cells treated with DCM leaf extract of V. glabra was studied using Hoechst 33342 dye and caspase colorimetry.
Results: Both DCM extracts of leaves and flowers possessed broad-spectrum APF activity against HT-29, MCF-7, MDA-MB-231 and SKOV-3 cancer cells. From DCM leaf extract, stearic acid (SA) and ursolic acid (UA) were isolated by column chromatography, and identified by NMR and MS analyses. APF activity of SA from DCM leaf extract displayed weak inhibitory activity and scientific literature showed UA has anticancer properties against those cancer cells used in this study. MDA-MB-231 cancer cells treated with DCM leaf extract and stained with Hoechst 33342 dye provided evidence that the extract had an apoptotic effect on the cells. Caspase colorimetry showed that the apoptotic effect involved activation of caspase-8, -9 and -3, but not caspase-6.
Conclusion: The potential of V. glabra as a candidate species for anticancer drugs warrants further investigation.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078344 | PMC |
http://dx.doi.org/10.4103/0973-1296.133238 | DOI Listing |
Sci Rep
January 2025
Section of Botany, Department of Biology, Science Faculty, Ege University, Bornova, İzmir, Turkey.
Despite its important pharmacological bioactivities, betulinic acid is still primarily obtained through extraction from heartwood and bark or synthesized synthetically, with less than 3% efficiency. Our endemic rose species, Rosa pisiformis (Christ.) D.
View Article and Find Full Text PDFPharmaceuticals (Basel)
November 2024
Laboratório de Produtos Bioativos (LPBio), Instituto de Ciências Farmacêuticas, Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé 27930-560, RJ, Brazil.
: Tuberculosis (TB) is one of the leading infectious causes of death worldwide, highlighting the importance of identifying new anti-TB agents. In previous research, our team identified antimycobacterial activity in leaf extract; therefore, this study aims to conduct further exploration of its potential. : Classical chromatography was applied for fractionation and spectrometric techniques were utilized for chemical characterization.
View Article and Find Full Text PDFFoods
November 2024
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
The incidence of multiple-organ cancers has recently increased due to simultaneous exposure to various environmental carcinogens. Thunb. () is recognized for its many health benefits, including its anti-cancer properties.
View Article and Find Full Text PDFBiology (Basel)
September 2024
Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar.
is a widely used plant in traditional Mediterranean and Middle Eastern medicine for various illnesses. has been shown to have anticancer effects against various cancers, but its effects against lung cancer have been under limited investigation. At the same time, is rich in terpenoids whose anti-lung cancer effects have been poorly investigated.
View Article and Find Full Text PDFJ Ethnopharmacol
January 2025
Instituto de Pesquisas de Produtos Naturais Walter Mors, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:
Ethnopharmacological Relevance: Ficus maxima is a medicinal plant extensively used in traditional medicine by Indigenous peoples across Central and South America. It is a member of the family Moraceae, subgenus Pharmacosycea, employed in treating various conditions, including intestinal parasites, gingivitis, internal inflammations, and snake bites. Despite its significant pharmacological potential, the species remains underrepresented in scientific literature.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.