AI Article Synopsis
- A new species of entomopathogenic nematode, Steinernema tophus n. sp., has been identified in South Africa using a combination of morphological and molecular techniques.
- Key diagnostic features for this species include specific characteristics of the spicules and gubernaculum, as well as measurable traits of infective juveniles.
- Molecular analysis, particularly of ribosomal DNA sequences, supports the classification of S. tophus n. sp. as a distinct species within the 'glaseri-group' of Steinernema.
Article Abstract
A new entomopathogenic nematode, Steinernema tophus n. sp. is described from South Africa. Morphological, molecular (ribosomal gene sequence data) together with cross-hybridization studies were used for diagnostics and identification purposes. Both molecular and morphological data indicate the new species belongs to the 'glaseri-group' of Steinernema spp. Key morphological diagnostic traits for S. tophus n. sp. include the morphology of the spicules and gubernaculum. Morphometric traits of third-stage infective juveniles, including total body length (average 1,046 µm), tail length (average 70 µm), location of the excretory pore (average 92 µm), D% (average 63), E% (average 132) and H% (average 32) values are definitive. In addition to these morphological characters, analysis of rDNA (28S and ITS) gene sequences depict this Steinernema species as a distinct and unique entity.
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Article Synopsis
- A new species of entomopathogenic nematode, Steinernema tophus n. sp., has been identified in South Africa using a combination of morphological and molecular techniques.
- Key diagnostic features for this species include specific characteristics of the spicules and gubernaculum, as well as measurable traits of infective juveniles.
- Molecular analysis, particularly of ribosomal DNA sequences, supports the classification of S. tophus n. sp. as a distinct species within the 'glaseri-group' of Steinernema.
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