Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178846 | PMC |
| http://dx.doi.org/10.1128/JVI.00745-14 | DOI Listing |
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