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A human skin-on-a-chip platform for microneedling-driven skin cancer treatment.
Mater Today Bio
February 2025
Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, CA, 90024, USA.
Skin-on-a-chip models provide physiologically relevant platforms for studying diseases and drug evaluation, replicating the native skin structures and functions more accurately than traditional 2D or simple 3D cultures. However, challenges remain in creating models suitable for microneedling applications and monitoring, as well as developing skin cancer models for analysis and targeted therapy. Here, we developed a human skin/skin cancer-on-a-chip platform within a microfluidic device using bioprinting/bioengineering techniques.
View Article and Find Full Text PDFImproved Skin Barrier Function Along with Hydration Benefits of Extract, Aesculin, and Schaftoside and LC-HRMS/MS Dereplication of Its Bio-Active Components.
Int J Mol Sci
November 2024
L'Oréal Research and Innovation, Bangalore 560067, India.
The skin hydration level is a key factor that influences the physical and mechanical properties of the skin. The stratum corneum (SC), the outermost layer of the epidermis, is responsible for the skin's barrier function. In this study, we investigated the role of a unique composition of extract for its ability to activate CD44, a cell-surface receptor of hyaluronic acid, and aquaporin-3, a water-transporting protein, in human keratinocytes (HaCaT).
View Article and Find Full Text PDFA photocurable and thermocurable composite hydrogel and the application in a contraction resistant full-thickness skin model.
Biofabrication
November 2024
Medical Engineering, Southeast University, Nanjing, Nanjing, 210096, CHINA.
Three-dimensional (3D) organotypic skin in vitro has attracted increasing attention for drug development, cosmetics evaluation, and even clinical applications. However, the severe contraction of these models restricts their application, especially in the analyses based on barrier functions such as percutaneous penetration. For the full-thickness skin equivalents, the mechanical properties of the dermis scaffold plays an important role in the contraction resistance.
View Article and Find Full Text PDFEpidermaQuant: Unsupervised Detection and Quantification of Epidermal Differentiation Markers on H-DAB-Stained Images of Reconstructed Human Epidermis.
Diagnostics (Basel)
August 2024
Department of Data Science and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland.
The integrity of the reconstructed human epidermis generated in vitro can be assessed using histological analyses combined with immunohistochemical staining of keratinocyte differentiation markers. Technical differences during the preparation and capture of stained images may influence the outcome of computational methods. Due to the specific nature of the analyzed material, no annotated datasets or dedicated methods are publicly available.
View Article and Find Full Text PDFNovel three-dimensional live skin-like in vitro composite for bioluminescence reporter gene assay.
FEBS J
October 2024
Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
We genetically manipulated HaCaT cells, a spontaneously immortalised normal keratinocyte cell line, to stably express two different coloured luciferase reporter genes, driven by interleukin 8 (IL-8) and ubiquitin-C (UBC) promoters, respectively. Subsequently, we generated a three-dimensional (3D) skin-like in vitro composite (SLIC) utilising these cells, with the objective of monitoring bioluminescence emitted from the SLIC. This SLIC was generated on non-woven silica fibre membranes in differentiation medium.
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