AI Article Synopsis
Article Abstract
Threose nucleic acid (TNA) is an unnatural genetic polymer composed of repeating threofuranosyl sugars linked by 2' and 3' phosphodiester bonds. TNA is capable of forming antiparallel Watson-Crick duplex structures in a self-pairing mode, and can also cross-pair opposite complementary strands of DNA and RNA. The solution NMR structure of a self-complementary TNA duplex reveals that TNA adopts an A-form helical structure, which explains its ability to exchange genetic information with natural genetic polymers. In a recent advance, a TNA aptamer was evolved from a pool of random sequences using an engineered polymerase that can copy DNA sequences into TNA. This unit details the steps required to evolve functional TNA molecules in the laboratory using a method called DNA display. Using this approach, TNA molecules are physically linked to their encoding double-stranded DNA template. By linking TNA phenotype with DNA genotype, one can select for TNA molecules with a desired function and recover their encoding genetic information by PCR amplification. Each round of selection requires ∼3 days to complete and multiple rounds of selection and amplification are required to generate functional TNA molecules.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/0471142700.nc0908s57 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
6-Trifluoromethylnicotinamide Bilaterally Anchored All Cations Implementing Efficient and Humidity-Stable Perovskite Solar Cells.
ACS Appl Mater Interfaces
November 2024
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education; Shaanxi Key Laboratory for Advanced Energy Devices; Shaanxi Engineering Lab for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, China.
For organic-inorganic hybrid perovskite solar cells (PSCs), the volatilization of organic components and the presence of undercoordinated Pb ions are the primary causes of device stability imbalance. These factors also serve as significant determinants that restrict the commercialization scale of PSCs. Here, 6-trifluoromethylnicotinamide (TNA) molecules, featuring amide and trifluoromethyl groups at both ends, were introduced as Lewis additives into the precursor solution of perovskite to anchor all cation defect sites and optimize the crystallization process of perovskite.
View Article and Find Full Text PDFTNA-Mediated Antisense Strategy to Knockdown Akt Genes for Triple-Negative Breast Cancer Therapy.
Small Methods
November 2024
Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China.
Triple-negative breast cancer (TNBC) remains a significant challenge in terms of treatment, with limited efficacy of chemotherapy due to side effects and acquired drug resistance. In this study, a threose nucleic acid (TNA)-mediated antisense approach is employed to target therapeutic Akt genes for TNBC therapy. Specifically, two new TNA strands (anti-Akt2 and anti-Akt3) are designed and synthesized that specifically target Akt2 and Akt3 mRNAs.
View Article and Find Full Text PDFUltra-stable threose nucleic acid-based biosensors for rapid and sensitive nucleic acid detection and in vivo imaging.
Acta Biomater
March 2024
Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR, P. R. China; Key Laboratory of Biochip Technology, Biotech and Health Care, Shenzhen Research Institute of City University of Hong Kong, 518057 Shenzhen, P. R. China. Electronic address:
The human genome's nucleotide sequence variation, such as single nucleotide mutations, can cause numerous genetic diseases. However, detecting nucleic acids accurately and rapidly in complex biological samples remains a major challenge. While natural deoxyribonucleic acid (DNA) has been used as biorecognition probes, it has limitations like poor specificity, reproducibility, nuclease-induced enzymatic degradation, and reduced bioactivity on solid surfaces.
View Article and Find Full Text PDFThreose nucleic acid as a primitive genetic polymer and a contemporary molecular tool.
Bioorg Chem
February 2024
State Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, Jiangsu 210023, China. Electronic address:
Nucleic acids serve a dual role as both genetic materials in living organisms and versatile molecular tools for various applications. Threose nuclei acid (TNA) stands out as a synthetic genetic polymer, holding potential as a primitive genetic material and as a contemporary molecular tool. In this review, we aim to provide an extensive overview of TNA research progress in these two key aspects.
View Article and Find Full Text PDFA Fast Na-Ion Conduction Polymer Electrolyte via Triangular Synergy Strategy for Quasi-Solid-State Batteries.
Angew Chem Int Ed Engl
December 2023
College of Chemistry & Green Catalysis Center, Zhengzhou University, Zhengzhou, 450001, Henan, P. R. China.
Polymer electrolytes provide a visible pathway for the construction of high-safety quasi-solid-state batteries due to their high interface compatibility and processability. Nevertheless, sluggish ion transfer at room temperature seriously limits their applications. Herein, a triangular synergy strategy is proposed to accelerate Na-ion conduction via the cooperation of polymer-salt, ionic liquid, and electron-rich additive.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!