Objective: This study aimed to investigate the molecular mechanism underlying the myeloperoxidase (MPO) induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Methods: HUVEC-12 cells were treated with myeloperoxidase at different concentrations (0.1 μ/ml, 0.2 μ/ml, 0.4 μ/ml and 0.6 μ/ml) and apoptotic cells were detected by flow cytometry. Then, cells were harvested for the detection of mRNA and protein expression of caspase-3 and Bax by reverse transcription PCR and Western blot assay, respectively.
Results: When compared with negative control group, the apoptosis index in 0.2 μ/ml MPO group, 0.4 μ/ml MPO group and 0.6 μ/ml MPO group increased markedly (P<0.05). When compared with negative control group, the mRNA expression of caspase-3 in 0.6 μ/ml MPO group and positive control group increased dramatically (P<0.05). When compared with negative control group, the protein expression of pre-caspase-3 and activated caspase-3 elevated significantly in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05). When compared with negative control group, the mRNA and protein expression of Bax elevated dramatically in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05).
Conclusion: MPO at certain extents may induce the apoptosis of HUVEC-12. The MPO induced apoptosis of HUVEC-12 may be dependent on capase-3 signaling pathway, and Bax is also involved in the MPO induced apoptosis of HUVEC-12.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057836 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Accuracy of continuous glucose monitoring in the hospital setting: an observational study.
Diabetologia
December 2024
Diabetes Research Center, Massachusetts General Hospital, Boston, MA, USA.
Aims/hypothesis: Continuous glucose monitoring (CGM) improves glycaemic outcomes in the outpatient setting; however, there are limited data regarding CGM accuracy in hospital.
Methods: We conducted a prospective, observational study comparing CGM data from blinded Dexcom G6 Pro sensors with reference point of care and laboratory glucose measurements during participants' hospitalisations. Key accuracy metrics included the proportion of CGM values within ±20% of reference glucose values >5.
The effects of pinealectomy and melatonin treatment in acrylamide-induced nephrotoxicity in rats: Antioxidant and anti-inflammatory mechanisms.
Physiol Behav
March 2024
Department of Medical Biochemistry, Faculty of Medicine, Histology Embryology, Karabuk University, Karabuk, Turkey.
Objective: Acrylamide (AA) is toxic and forms in food that undergoes high-temperature processing. This study aimed to investigate the effects of AA-induced toxicity on renal tissue in pinealectomized rats and the possible protective effect of exogenous Melatonin (ML) administration.
Materials And Methods: Sixty rats were randomized into 6 groups (n = 10): Sham, Sham+AA, Sham+AA+ML, PX, PX+AA, and PX+AA+ML.
Screening of Phytochemical, Antimicrobial, and Antioxidant Properties of from Northeastern Morocco.
Life (Basel)
October 2023
Laboratoire d'Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, Morocco.
, acknowledged through its indigenous nomenclature "samar", is part of the Juncaceae taxonomic lineage, bearing considerable import as a botanical reservoir harboring conceivable therapeutic attributes. Its historical precedence in traditional curative methodologies for the alleviation of infections and inflammatory conditions is notable. In the purview of Eastern traditional medicine, Juncus species seeds find application for their remedial efficacy in addressing diarrhea, while the botanical fruits are subjected to infusion processes targeting the attenuation of symptoms associated with cold manifestations.
View Article and Find Full Text PDFUnilateral Vocal Fold Paralysis With Large Posterior Glottic Gap: Is Arytenoid Procedure Necessary?
Ann Otol Rhinol Laryngol
August 2022
Department of Otolaryngology-Head and Neck Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey.
Objectives: For unilateral vocal fold paralysis (UVFP) with large posterior glottic gap medialization laryngoplasty (ML) + arytenoid adduction (AA), ML + adduction arytenopexy (AApexy), and ML alone using prosthesis with posterior extension are possible solutions. This study was carried out to elucidate the controversy among these solution options.
Methods: Retrospective cohort.
First evaluation of genotoxicity of strong bases and zwitterions in treated household effluents.
J Hazard Mater
August 2021
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Article Synopsis
- Various genotoxic substances in household waste have not been thoroughly researched, prompting a study to evaluate their effects using the umu test and solid-phase extraction based on acid-base properties.
- Results indicated that substances with acidic functional groups exhibited higher genotoxicity, with significant measurements recorded both with and without an S9 enzyme, while basic substances showed some toxicity, albeit less severe.
- Ion-exchange cartridges indicated that strong acid functional groups lacked genotoxicity, and the genotoxicity levels of weak acids and neutral substances were generally lower, highlighting the first investigation of zwitterions' genotoxicity in effluents.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!