AI Article Synopsis
- The cps-1 gene in Neurospora crassa encodes a polysaccharide synthase similar to the Cps1p in Cryptococcus neoformans and is found in various fungi.
- Deleting the cps-1 gene leads to defects in the cell wall that disrupt all stages of N. crassa's life cycle, affecting growth and reproductive structures.
- CPS-1 is essential for proper cell wall protein incorporation and overall cell wall structure, and it does not produce hyaluronic acid in N. crassa.
Article Abstract
The Neurospora crassa cps-1 gene encodes a polysaccharide synthase with homology to the Cryptococcus neoformans hyaluronic acid synthase Cps1p. Homologs of the cps-1 gene are found in the genomes of many fungi. Loss of CPS-1 results in a cell wall defect that affects all stages of the N. crassa life cycle, including vegetative growth, protoperithecia (female mating structure) development, and conidia (asexual spore) development. The cell wall of cps-1 deletion mutants is sensitive to cell wall perturbation reagents. Our results demonstrate that CPS-1 is required for the incorporation of cell wall proteins into the cell wall and plays a critical role in cell wall biogenesis. We found that the N. crassa cell wall is devoid of hyaluronic acid, and conclude that the polysaccharide produced by the CPS-1 is not hyaluronic acid.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115004 | PMC |
| http://dx.doi.org/10.1016/j.fgb.2014.05.009 | DOI Listing |
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