AI Article Synopsis
- A new detection method for tobacco mosaic virus (TMV) in irrigation water has been developed, allowing reliable identification at low concentrations (10 viral copies/μL) using real-time PCR.
- The detection sensitivity improved when polyethylene glycol 6000 (PEG6000) was used to concentrate TMV from water samples, allowing 17 out of 28 samples from Shaanxi Province to test positive.
- This method confirms the infectivity of TMV and sets a threshold for re-infection at 10² viral copies/mL, providing an effective tool for managing crop infections.
Article Abstract
Tobacco mosaic virus (TMV) causes significant yield loss in susceptible crops irrigated with contaminated water. However, detection of TMV in water is difficult owing to extremely low concentrations of the virus. Here, we developed a simple method for the detection and quantification of TMV in irrigation water. TMV was reliably detected at concentrations as low as 10 viral copies/μL with real-time PCR. The sensitivity of detection was further improved using polyethylene glycol 6000 (PEG6000, MW 6000) to concentrate TMV from water samples. Among the 28 samples from Shaanxi Province examined with our method, 17 were tested positive after virus concentration. Infectivity of TMV in the original water sample as well as after concentration was confirmed using PCR. The limiting concentration of TMV in water to re-infect plants was determined as 10(2) viral copies/mL. The method developed in this study offers a novel approach to detect TMV in irrigation water, and may provide an effective tool to control crop infection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206383 | PMC |
| http://dx.doi.org/10.1007/s12250-014-3461-7 | DOI Listing |
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