The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699287 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-0944-5_28 | DOI Listing |
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