An immunosensor for the rapid detection of 1,3-dinitrobenzene was developed based on an evanescent wave all-fiber biosensing platform with the detection limits of 0.054 mg x L(-1), and the detection cycle was less than 10 min. Hapten-carrier conjugates NB-OVA were synthesized by mixing 4-nitrohippuric acid and OVA activated by EDC, and then the conjugates were immobilized onto the silane layer on the probe with a heterobifunctional crosslinker. The probe modified had good robustness and regeneration performance, which allowed the performance of more than 100 assay cycles without significant loss of reactivity. Several water samples of different origins were measured with less than 4.5% -10.0% deviation of the detection and the recovery rate of 1,3-dinitrobenzene was between 80% and 120%, which proved the system's precision and accuracy and negligible matrix effects. This immunosensor shows great potential in rapid detection of 1,3-dinitrobenzene in practical waters.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Temporal Unfolding of Spelling-to-sound Mappings in Visual (Non)word Recognition.
J Cogn Neurosci
January 2025
Universidade de Lisboa, Lisbon, Portugal.
Behavioral research has shown that inconsistency in spelling-to-sound mappings slows visual word recognition and word naming. However, the time course of this effect remains underexplored. To address this, we asked skilled adult readers to perform a 1-back repetition detection task that did not explicitly involve phonological coding, in which we manipulated lexicality (high-frequency words vs.
View Article and Find Full Text PDFBeyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.
Vet Med Sci
January 2025
Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Background: Brucellosis is a zoonotic disease caused by Brucella spp., affecting various animals and humans, leading to significant economic and public health impacts. Traditional diagnostic methods, mainly serological, often fail to detect seronegative carriers, which continue to spread the infection.
View Article and Find Full Text PDFSmartphone-assisted hydrogel sensing platform based on double emission carbon dots for portable on-site tetracycline detection.
Mikrochim Acta
January 2025
Department of Chemistry and Material Engineering, Lyuliang University, Lyuliang, 033000, P. R. China.
Innovative double-emission carbon dots (DE-CDs) were synthesized via a one-step hydrothermal method using fennel and m-phenylenediamine (m-PD) as precursors. These DE-CDs exhibited dual emission wavelengths at 432 and 515 nm under different excitations, making them highly versatile for fluorescence-based applications. The fluorescence of the DE-CDs was efficiently quenched by tetracycline (TC) through the inner filter effect (IFE), allowing for the construction of a sensitive dual-response fluorescent sensor.
View Article and Find Full Text PDFUltrasensitive "On-Off" Ratiometric Fluorescence Biosensor Based on RPA-CRISPR/Cas12a for Detection of .
J Agric Food Chem
January 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
() is a major pathogenic bacterium responsible for bacterial foodborne diseases, making its rapid, specific, and accurate detection crucial. In this study, we develop a ratiometric biosensor based on the recombinase polymerase amplification-clustered regularly interspaced short palindromic repeats/CRISPR associated protein 12a (RPA-CRISPR/Cas12a) system and Eu-metal-organic framework (Eu-MOF) fluorescent nanomaterials for the high-sensitivity detection of , combining with RPA for efficient isothermal amplification, this sensor enhances specificity and sensitivity by utilizing the target activation of CRISPR/Cas12a. The Eu-MOF serves a dual function, providing stable red fluorescence as a reference signal and adsorbing FAM-labeled probes for fluorescence quenching, forming a dual-signal system that significantly reduces background interference.
View Article and Find Full Text PDFThe Complete Blood Count Sepsis Index Using Monocyte Distribution Width for Early Detection of Sepsis in Patients Without Obvious Signs.
Crit Care Explor
January 2025
Department of Pediatrics, Johns Hopkins University, Baltimore, MD.
Objectives: Exploiting the complete blood count (CBC) with differential (CBC-diff) for early sepsis detection has practical value for emergency department (ED) care, especially for those without obvious presentations. The objective of this study was to develop the CBC Sepsis Index (CBC-SI) that incorporates monocyte distribution width (MDW) to enhance rapid sepsis screening.
Design: A retrospective observational study.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!